Nature 2020 01 30 Part.02

712 | Nature | Vol 577 | 30 January 2020 Article which was absent in a separate map of SAGA lacking TBP (Extended Data Fig. 3a–d ...

Nature | Vol 577 | 30 January 2020 | 713 The Spt3 deviation from the canonical octamer does not originate from a single structur ...

714 | Nature | Vol 577 | 30 January 2020 Article Extended Data Fig. 7a) of DNA binding to SAGA–TBP demonstrate that this mild st ...

Nature | Vol 577 | 30 January 2020 | 715 DUB-anchoring domain traverses the core, entering proximal to the Taf9 histone fold and ...

716 | Nature | Vol 577 | 30 January 2020 Article Lee, K. K. et al. Combinatorial depletion analysis to assemble the network arc ...

Methods No statistical methods were used to predetermine sample size. Purification of SAGA The SAGA complex was purified from nu ...

Article CGTA. The position of the TATA sequence is indicated by bold letters and the two mismatches in the TATA-less sequences a ...

For a large proportion of the side chains, density was clear and practi- cally all densities in the core of SAGA and Tra1 could ...

Article Extended Data Fig. 1 | Subunit and domain organization of the yeast SAGA complex. a, SAGA subunits are organized into th ...

Extended Data Fig. 2 | Cryo-EM data-analysis strategy and resolution assessment of the cryo-EM structures. a, Image-processing s ...

Article Extended Data Fig. 3 | Fitting of TBP and Spt8 into the cryo-EM map. a, Pairwise alignment of TBP from P. pastoris and S ...

Extended Data Fig. 4 | Comparing the arrangement of common SAGA and TFIID subunits. a, The Taf5 NTD and Taf6 HEAT repeats have d ...

Article Extended Data Fig. 5 | Interactions between histone-fold pairs in SAGA deviate from the nucleosome pattern already at th ...

Extended Data Fig. 6 | Mapping previously isolated mutations in Spt3 and T B P. Several classes of Spt3 and TBP mutations were s ...

Article Extended Data Fig. 7 | TFIIA-dependent TBP release from SAGA. a, Experimental set-up for pull-down assays that investiga ...

Extended Data Fig. 8 | Flexibility and anchoring of the enzymatic modules. a, Two-dimensional classes of SAGA side views reveali ...

Article Extended Data Fig. 9 | Protein loops and tails contribute to an intricate network of interactions and to bridges between ...

Extended Data Table 1 | Cryo-EM data collection, refinement and validation statistics *In addition a model including Spt8 and DU ...

1 nature research | reporting summary October 2018 Corresponding author(s): Schultz, Ben-Shem Last updated by author(s):Nov 4, 2 ...

2 nature research | reporting summary October 2018 For a reference copy of the document with all sections, see nature.com/docume ...