Nature 2020 01 30 Part.02

682 | Nature | Vol 577 | 30 January 2020 Article Host-mediated ubiquitination of a mycobacterial protein suppresses immunity Lin ...

Nature | Vol 577 | 30 January 2020 | 683 cells during M. tuberculosis infection^5 ; TH1 cells produce interferon (IFN)γ; and IFN ...

684 | Nature | Vol 577 | 30 January 2020 Article of Il1b and Il6 in macrophages infected with H37RvΔRv0222 (Extended Data Fig. 2 ...

Nature | Vol 577 | 30 January 2020 | 685 interacts with TRAF6, we next examined whether Rv0222 is ubiqui- tinated in host cells. ...

686 | Nature | Vol 577 | 30 January 2020 Article 0 50 100 150 Fold change inIl12 p mRNA P = 0. P = 0. P = 0. 130 0 2 4 6 8 log [ ...

Nature | Vol 577 | 30 January 2020 | 687 complemented with Rv0222, but not with Rv0222(K76A), induced much lower expression of p ...

688 | Nature | Vol 577 | 30 January 2020 Article Alonso, S., Pethe, K., Russell, D. G. & Purdy, G. E. Lysosomal killing of ...

Methods Bacterial strains and cells Bacterial strains are described in Supplementary Table 1. Mycobacterial strains were grown i ...

Article Mice and infection All animal experiments were reviewed and approved by the Animal Experiment Administration Committee o ...

Extended Data Fig. 1 | Rv0222 inhibits host inf lammatory responses. a, b, Luciferase assay of HEK293T cells transfected with th ...

Article Extended Data Fig. 2 | See next page for caption. ...

Extended Data Fig. 2 | Rv0222 interacts with and inhibits TR AF6 signalling. a, Immunoblot of lysates from peritoneal macrophage ...

Article Extended Data Fig. 3 | Rv0222 is K11-ubiquitinated at K76 by host ANAPC2. a, b, Immunoblots and immunoprecipitates of HE ...

Extended Data Fig. 4 | See next page for caption. ...

Article Extended Data Fig. 4 | K11-linked ubiquitination of Rv0222 by ANAPC suppresses TR AF6 signalling. a, b, Luciferase assay ...

Extended Data Fig. 5 | K11-linked ubiquitination of Rv0222 by ANAPC suppresses anti- tuberculosis immunity. a, Survival of six-w ...

Article Extended Data Fig. 6 | Summary diagram. The K11-linked ubiquitination (K11ub) of M.tuberculosis-secreted protein Rv0222 ...

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