Science - 06.12.2019

Trimer Immunization Using Optimized Approaches.Immunity 46 , 1073–1088.e6 (2017). doi:10.1016/j.immuni.2017.05.007; pmid: 286369 ...

RESEARCH ARTICLE SUMMARY ◥ HIV VACCINES A generalized HIV vaccine design strategy for priming of broadly neutralizing antibody r ...

RESEARCH ARTICLE ◥ HIV VACCINES A generalized HIV vaccine design strategy for priming of broadly neutralizing antibody responses ...

vaccine targeting, namely that the high mutation level in BG18 itself is not required to achieve substantial neutralization brea ...

capable of binding and priming a broad range of BG18-like precursor B cells. Seven Env mammalian cell-surface display libraries, ...

development, we tested whether day 42 anti- bodies could bind Env trimers more native- like than the germline-targeting immunoge ...

further examination of the biochemical proper- ties and specificities of the naïve Abs. Twenty- three HMP Fabs bound to N332-GT1 ...

owing to the structural requirements for the bnAbHCDR3toreachtheHIVEnvprotein surface at the base of V3 while avoiding V1 loop g ...

Steichenet al.,Science 366 , eaax4380 (2019) 6 December 2019 7of13 HCDR3 AKDFLLSTSGYLSYGDYYYYGMDV ARSWALFCSSTSCTNYYYYYGMDV TTDIG ...

bnAbs, we solved high-resolution cryo-EM structures of the complexes of N332-GT5 bound to HMP1 (type I) and HMP42 (type II), wit ...

Replicates Data presented are from two independent ex- periments. The results have been reproduced in at least two additional ex ...

anti-CMYC (ICL inc). Typically, libraries were sorted three to five times, and the enriched cell populations were frozen until s ...

3 min followed by a 5-min dissociation time. Regeneration was accomplished using 3 M magnesium chloride with a 180-s contact tim ...

(PE-Cy7,ThermoFisher, clone HIB19) and anti- CD20 (PE-Cy7, ThermoFisher, clone 2H7), in addition to anti-IgG (APC-Cy7, Biolegend ...

J. G. Jardineet al., Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen. Science 34 ...

RESEARCH ARTICLE SUMMARY ◥ ASTEROIDS Episodes of particle ejection from the surface of the active asteroid (101955) Bennu D. S. ...

RESEARCH ARTICLE ◥ ASTEROIDS Episodes of particle ejection from the surface of the active asteroid (101955) Bennu D. S. Lauretta ...

later shows objects in common with the earlier image that have moved away from Bennu, implying the movement of discrete particle ...

Characterization of the largest observed events For the 6 January, 19 January, and 11 February events, a particle distribution p ...

Particle properties We constrained the area-to-mass ratios (where area is cross-sectional) of the six bound par- ticles by using ...