Nature - USA (2019-07-18)
reSeArcH Article with disease-modifying therapy at the time of biopsy (Supplementary Table 1). CALR genotyping data were availab ...
Article reSeArcH high expression of MkP-related genes^26 (HSClowMkPhigh) (Fig. 2e, Supplementary Table 2). Even within the MkP c ...
reSeArcH Article CALR mutations extends to uncommitted progenitors (Fig. 3b). This is further supported by the upregulation of A ...
Article reSeArcH (that is, the triple mutant) did not further increase cell-cycle activation. Thus, multiplexed GoT demonstrates ...
reSeArcH Article site of XBP1 to demonstrate that IRE1 activity was increased in the mutant cells, including HSPCs. Our data thu ...
Article reSeArcH Methods No statistical methods were used to predetermine sample size. The experiments were not randomized and i ...
reSeArcH Article were aligned to the human reference sequence GRCh38 or hg19, or to mouse refer- ence mm10 (species-mixing exper ...
Article reSeArcH using the logistic regression for differential gene expression^55 , with variable genes as input and requiring ...
reSeArcH Article Oncogene TSG 0 0.2 0.4 0.6 0.8 1 00 .2 0.40.6 0.81 VAF in WES I II III IV V VAF in RNAseq WES’s VAF = 0 RNA-seq ...
Article reSeArcH Barcode UMI Primer Shared Target (R1.fastq) (R2.fastq) Linear amplification GoT reads Barcode UMI Primer Shared ...
reSeArcH Article Number of UMI for reads with CALR locus of interest 01234 >5 ET01 10X 3’ v2 GoT MF01 10X 3’ v2 GoT ET05 10X ...
Article reSeArcH ab # cells = 18,722 Patient ID ET01 ET02 ET03 ET04 ET05 Relative expression Low CD79A DNTT IRF7 ELANEAZU1 MPO S ...
reSeArcH Article ET02 ET03 ET05 ET04 ET01 0 0.5 1 Mutant cell ratio Minimum CALR UMI threshold Pseudotime 10 20 30 40 CD34+ CD10 ...
Article reSeArcH ET03 ET04 ET05 ET01 ET02 Log (S phase module) 2 ●●●●●● ●● ●● ●● ●● ●●●● ●● ●● ●● ●● ●● ●● ●● ●● ●● ●● ●●●● ●●●● ...
reSeArcH Article HSPC WT HSPC MUT MkP WT MkP MUT (n = 19) (n = 16) (n = 17) (n = 51) (n = 108) (n = 240) (n = 25) (n = 276) (n = ...
Article reSeArcH MF01 MF02 MF03 MF04 HSPC WT HSPC MUT MkP WT MkP MUT HSPC MkP MEP Log (Cell cycle module) 2 3 4 5 6 P = 0.66 3 4 ...
reSeArcH Article (i) 50 bases at 5’ end mapped to SF3B1 (ii) 50 bases at 5’ end mapped to other transcripts (ii) 2.2% 5’ 3’ (i) ...
Article reSeArcH 0 5 10 15 Log (Number of supporting reads per barcode) 2 0 10 20 30 Base quality at the differing base 10 20 13 ...
1 nature research | reporting summary April 2018 Corresponding author(s): Dan Landau Reporting Summary Nature Research wishes to ...
2 nature research | reporting summary April 2018 Data Policy information about availability of data All manuscripts must include ...
«
1
2
3
4
5
6
7
8
9
10
»
Free download pdf