Nature - USA (2020-10-15)

Article Extended Data Fig. 4 | Structural features of the SKP1/FBXL17-BTB complex. a, Elution profile of the SKP1/FBXL17-BTB(KEA ...

Extended Data Fig. 5 | See next page for caption. ...

Article Extended Data Fig. 5 | Validation of SKP1/FBXL17-BTB structure through FBXL17 mutations. a, Single mutations of FBXL17 r ...

Extended Data Fig. 6 | Validation of SKP1/FBXL17-BTB structure through KEAP1 mutations. a, Single mutations in KEAP1 do not inhi ...

Article Extended Data Fig. 7 | Sequence alignment of BTB domains. Residues involved in BTB dimerization are marked by a blue dot ...

Extended Data Fig. 8 | Binding and destabilization of BTB dimers by SCFFBXL17. a, A FRET-based assay to monitor BTB dimer format ...

Article Extended Data Fig. 9 | The N-terminal β-strand is important for BTB complex formation and recognition. a, Chimeric KLHL1 ...

Extended Data Fig. 10 | The amino-terminal β-strand of BTB domains serves as a molecular barcode for functional dimerization. Se ...

Article Extended Data Table 1 | Data collection and refinement statistics for crystal structures Table shows crystallography ref ...

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Nature | Vol 586 | 15 October 2020 | 457 Article Structural basis for pH gating of the two-pore domain K + channel TASK2 Baobin ...

458 | Nature | Vol 586 | 15 October 2020 Article two extracellular cap-forming helices (CH1 and CH2) (Fig. 1f). The extracellula ...

Nature | Vol 586 | 15 October 2020 | 459 chain was most important for sealing the cavity with K245. Indeed, a smaller side chain ...

460 | Nature | Vol 586 | 15 October 2020 Article axis at low pH, N243 remains relatively stationary in closed and open structure ...

Nature | Vol 586 | 15 October 2020 | 461 We evaluated the pH sensitivity of TASK2 mutants to test the struc- tural model for gat ...

462 | Nature | Vol 586 | 15 October 2020 Article acknowledgements, peer review information; details of author con- tributions an ...

Methods Cloning, expression and purification Cloning, expression and purification were performed similarly to that described for ...

Article For TASK2 in nanodiscs at pH 6.5, 3,024 micrographs were collected and the data processing procedure used was similar as ...