Nature - USA (2020-09-24)

Article Extended Data Fig. 10 | AtPep1-induced decrease in stomatal conductance is impaired in osca1.3/1.7. Leaf transpiration w ...

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574 | Nature | Vol 585 | 24 September 2020 Article Homeostatic mini-intestines through scaffold-guided organoid morphogenesis Mi ...

Nature | Vol 585 | 24 September 2020 | 575 Establishment of tissue homeostasis Conventional epithelial organoids can be propagat ...

576 | Nature | Vol 585 | 24 September 2020 Article enteroendocrine cells (Fig. 2e) and goblet cells (Fig. 2f) were found almost ...

Nature | Vol 585 | 24 September 2020 | 577 of Goblet cells was much lower in both in vitro conditions (around 7% in vivo; 1% in  ...

578 | Nature | Vol 585 | 24 September 2020 Article experiments reveal that our bioengineered organoids show notable regenerative ...

Methods Data reporting No statistical methods were used to predetermine sample size. The experiments were not randomized. The in ...

Article ml−1 FLT3); differentiation day 12 and 14 (10 ng ml−1 hbFGF, 50 ng ml−1 SCF, 20 ng ml−1 IL-3, 20 ng ml−1 TPO and 20 ng m ...

formulation) was injected into the hydrogel compartment of the micro- device through the hydrogel-loading port and incubated at ...

Article for 15 min at 37 °C and seeded into the microdevices as described above for mouse ISCs. Non-adherent cells were washed a ...

Cell Ranger v.3.0.1. Raw count matrices were imported in Seurat 2.3.4 (ref.^37 ) using RStudio 1.1.463 (www.rstudio.com), and si ...

Article inlet reservoir. In the following days, mini-guts were cultured as previ- ously described, and perfusion was done manual ...

Nakamura, Y., Kimura, S. & Hase, K. M cell-dependent antigen uptake on follicle- associated epithelium for mucosal immune s ...

Article Extended Data Fig. 1 | Bioengineering intestinal stem cell epithelia with a tubular, in-vivo-like architecture. a, Photo ...

Extended Data Fig. 2 | Establishment of shape-controlled organoid culture from a variety of epithelial stem and progenitor cells ...

Article Extended Data Fig. 3 | Establishment of long-term culture and in vitro tissue homeostasis. a, Bright-field (middle) and ...

Extended Data Fig. 4 | Mini-gut tubes undergo rapid cell turnover and comprise key functional intestinal cell types. a, Epitheli ...