Nature - USA (2020-09-24)

Nature | Vol 585 | 24 September 2020 | 591 Article The liver–brain–gut neural arc maintains the Treg cell niche in the gut Toshi ...

592 | Nature | Vol 585 | 24 September 2020 Article FOXP3+ T helper cells, in particular Helios−RORγt+ pTreg cells, in the colon ...

Nature | Vol 585 | 24 September 2020 | 593 colonic APCs obtained from wild-type mice and human intestine (Fig. 1k, l), whereas c ...

594 | Nature | Vol 585 | 24 September 2020 Article characterized the effects of HVx on the gut and the spleen. We observed a sig ...

Nature | Vol 585 | 24 September 2020 | 595 wild-type HVx mice, but not mAChR TKO mice, treated with bethane- chol exhibited an i ...

596 | Nature | Vol 585 | 24 September 2020 Article Pavlov, V. A. & Tracey, K. J. Neural regulation of immunity: molecular m ...

Methods Animals C57BL/6 (WT) mice, BALB/c mice and Jcl:Wistar rats were purchased from Japan CLEA. Five-week-old male germ free ...

Article injection, mice were perfused with PBS and then with 4% PFA in PBS. Isolated NG and DRG were post-fixed for 2 h and cryo ...

We visualized the expression levels of APC subset signature genes with neurotransmitter receptor genes (expression >1 TPM in ...

Article according to the manufacturer’s protocol. Subsequently, male WT mice (weighing 22–25 g) were intravenously injected via ...

Extended Data Fig. 1 | See next page for caption. ...

Article Extended Data Fig. 1 | Muscarinic signalling in colonic APC activates induction of Treg. a, Three-dimensional reconstruc ...

Extended Data Fig. 2 | See next page for caption. ...

Article Extended Data Fig. 2 | Colitis activates liver-brain axis. a–c, WT mice were subjected to Sham or VGx and then were give ...

Extended Data Fig. 3 | Anatomy of the hepatic vagus nerve in mice. a, Anatomical diagram. b, Operative field for hepatic vagotom ...

Article Extended Data Fig. 4 | See next page for caption. ...

Extended Data Fig. 4 | Effects of vagotomy on maintenance and stability of colonic pTreg. WT mice were subjected to Sham, VGx or ...

Article Extended Data Fig. 5 | See next page for caption. ...

Extended Data Fig. 5 | Afferent vagal, but not spinal cord, activation from the liver is involved in colonic Treg homeostasis. a ...

Article Extended Data Fig. 6 | See next page for caption. ...