Plant Biotechnology and Genetics: Principles, Techniques and Applications

seems to be ideal for many cultures since it has all the nutrients that plants require for growth and contains them in the prope ...

Nitsch and Nitsch (1969) developed an another culture. Knudson’s medium (Knudson 1946) was developed for orchid tissue culture a ...

(indoleacetic acid), IBA (indolebutyric acid), NAA (naphthaleneacetic acid), and 2,4-D (2,4-dichlorophenoxyacetic acid). Cytokin ...

5.4 Sterile Technique 5.4.1 Clean Equipment Successful tissue culture requires the maintenance of a sterile environment. All tis ...

sterilant can improve surface contact with the tissue. Although surface contamination can be eliminated by sterilization, it is ...

photoperiod is optimal for tissue cultures, and a temperature of 22–25 8 C is used in most laboratories. A light intensity of 25 ...

Figure 5.7.(a) Callus tissue; (b,c) shoots arising from callus (an example of organogenesis). See color insert. Figure 5.8.Somat ...

application of callus is to inducesomaclonalvariation through which desired mutants can be selected. 5.6.1.1. Somaclonal Variati ...

are also advantageous for molecular farming because of high level of containment that they offer relative to whole, field-grown ...

hybrids as described below. Cell walls can be removed from explant tissue mechanically or enzymatically; the latter is used most ...

1975; Brown et al. 1988; Ayabe and Sumi 2001). Apical meristems in plants are suitable explants for the production of virus-free ...

Multiple-bud initiation still remains a challenge in many tree species since many tree species are recalcitrant in tissue cultur ...

5.7.2.1. Synthetic Seeds. Encapsulated somatic embryos are known assynthetic seeds. Somatic embryos are typically encapsulated i ...

conditions. With this method, acclimation of the rooted shoots can be carried out simultaneously. In vitro rooting consists of r ...

LIFE BOX 5.1. MARTHA S. WRIGHT Martha S. Wright, Research Scientist (retired), Syngenta and Monsanto Martha Wrightwith a regener ...

Eventually, several crops were in the race to the field. I left Monsanto in 1987 and started working at then Ciba- Geigy, now Sy ...

junior years of college when I got a job working for a plant breeder. I worked for the same breeder during the remainder of my b ...

References Ainsley PJ, Aryan AP (1998): Efficient plant regeneration system for immature embryos of triticale (x Triricosecale W ...

Knudson L (1946): A new nutrient solution for germination of orchid seed.Am Orch Soc Bull 15 :214–217. Kwon TH, Kim YS, Lee JH, ...

Wang L, Wang G, Hong N, Tang R, Deng X, Zhang H (2006): Effect of thermotherapy on elimination of apple stem grooving virus and ...