Plant Biotechnology and Genetics: Principles, Techniques and Applications

LIFE BOX 6.2. TONY CONNER Tony Conner, Senior Scientist, New Zealand Institute for Crop & Food Research; Professorial Fellow ...

This eventually led to our development of intragenic vector systems, which involve identifying functional equiva- lents of vecto ...

Kankel MW, Ramsey DE, Stokes TL, Flowers SK, Haag JR, Jeddeloh JA, Riddle NC, Verbsky ML, Richards EJ (2003): Arabidopsis MET1 c ...

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&CHAPTER 7 Recombinant DNA, Vector Design, and Construction MARK D. CURTIS Institute of Plant Biology, University of Zurich, ...

7.1 DNA Modification Recombinant DNA technology relies on the ability to manipulate DNA using nucleic acid– modifying enzymes. T ...

until a year later in 1973 that Stanley Cohen and Herbert Boyer created the first genetically modified organism using these appr ...

DNA molecules to a host cell for amplification is achieved in a process known astransform- ation. Observations in the late 1920s ...

7.2 DNA Vectors In molecular biology, a cloning vector is a DNA molecule that carries foreign DNA fragments into a host cell and ...

replication is either “relaxed” or “stringent,” a characteristic determined by the origin of replication. Plasmids with stringen ...

Figure 7.4.DNA fragments produced with a singleEcoRI restriction enzyme give rise to compatible protruding termini that can anne ...

effects at high copy number. Unlike chromosomal DNA, plasmid DNA is dispensable to the host, so why does the host keep it? To be ...

engineered plant cell. All the phyto-oncogenes (tumor-inducing genes) have been removed (Fig. 7.7). These plant vectors are know ...

expression and plant transformation (for more details, see Chapters 6 and 10). Failure to obtain gene expression using cistrons ...

The efficiency of transgene expression in plants is dependent on a number of factors that affect mRNA accumulation and stability ...

as a binding site for the heatshock protein, HSP101, which is required for translational enhancement. The efficiency of translat ...

sites that make them incompatible with some vectors. The development of the polymerase chain reaction (PCR) (Fig. 7.10) in 1985, ...

genomics studies that have resulted from the availability of whole-genome sequences. These novel cloning strategies rely onsite- ...

Excisionase (Xis) proteins (LR clonase), derived from elements used during the bacterio- phagellife cycle. DNA fragments flanked ...

reaction, to pDEST vectors containingattR1andattR2sites. The resulting recombinant DNA constructs are known as “expression clone ...