9780521516358book.pdf

related directly to an amino acid residue because the two flanking Y ions result from cleavage of two adjacent amide bonds. Ther ...

sample superimposes with its differentially labelled identical counterpart in the other sample. Scanning of the gel at two diffe ...

done using programs such as BLAST and PSI-BLAST. If sequence homology is found with a protein of known function, either from the ...

8.5.4 Protein–protein interactions Given the complex network of pathways that exist in the cell (signalling pathways, biosynthet ...

interacting proteins are being identified in individual organisms. This has led to the development of the Database of Interactin ...

the array surface for a period of time, then washed off, and the position(s) where the protein has bound, identified (see below) ...

analytical techniques such as mass spectrometry (see Chapter 9) and invariably requiring the interrogation of protein and genome ...

of a dynamic interacting system. This has led to the development of the concept of systems biology, which can be defined as the ...

9 Mass spectrometric techniques A. AITKEN 9.1 Introduction 9.2 Ionisation 9.3 Mass analysers 9.4 Detectors 9.5 Structural inform ...

has no units. However,Mris numerically equivalent to the mass,M, which does have units and the Dalton is frequently used (see Se ...

9.1.3 Vacuum system All mass analysers operate under vacuum in order to minimise collisions between ions and air molecules. With ...

substance (to produce a cation) or electron capture (to produce an anion). The analyte must be in the vapour state in the electr ...

precursor ion disintegrating into a number of smallerfragment ionsthat may be relatively unstable and further fragmentation may ...

atoms (such as Ar, He, Xe) of high velocity. A later development was the use of a beam of caesium (Csþ)ionsandthetermliquid seco ...

positiveandnegative ion mass spectrato be determined. The term pseudomolecular implies the mass of the ion formed from a substan ...

to high accuracy. The peaks can bedeconvolutedand presented as a single peak representing theMr(in this exampleM¼102658). A diag ...

is really measured is the mass-to-charge ratio (m/z) for each ion.) At any given moment, ions of a particular mass are allowed t ...

Quadrupole rods Ion that is unsuccessful at reaching detector Detector RF supply DC supply + + Ions Fig. 9.5Quadrupole ana ...

9.3.3 Ion trap mass spectrometry Ion trap mass spectrometers use ESI to produce ions, all of which are transferred into and subs ...

Ion trap MS permits structural information to be readily obtained (and sequence information in the case of polypeptides). Not on ...