Methods in Molecular Biology • 16 Enzymes of Molecular Biology

172 Pingoud, Alves, and Geiger °~ N o u 0 = % = E Z (^0) < ~J (^0) < e~ oO 0 rc o o r,D 8., k) k~ 0 ~D rc .= O ~Z 8., k~ . ...

Restriction Enzymes 173 0 © .v 0 z E ,.Q O e~ e-, r..) ,_~ ~0 o,.~ ~'~ Me "u ...

174 Pingoud, Alves, and Geiger 3.8. "Star"Activity "Star" activity, discussed in Section 2.4.2., is defined as a relaxed speci- ...

Restriction Enzymes Table 7 Isoschizomer Pairs that Can Be Used in the Analysis of the Methylation State of DNA 175 Methylated s ...

176 Pingoud, Alves, and Geiger Table 8 Restriction Enzymes Recognizing Palindromic Tetra- and Hexanucleotide Sequences a oo~ A~r ...

Restriction Enzymes Table 9 Restriction Enzymes Recognizing Palindromic Penta-, Hepta-, and Octanucleotide Sequences 177 Recogni ...

178 Pingoud, Alves, and Geiger *dO o 0 o e~ o o f=u o f2~ 0 Z Z Z Z r~ (.9 ÷ E 0 N "r. N 0 0 e- t~ ll ...

Restriction Enzymes 179 o e- ¢. °~^0 o i o o e. °~ OI o~ (^0) o f2U 0 0 (^0 0) 00<~0000000000 - -. _ -... o 00000000<~ 0 ...

(^180) Pingoud, Alves, and Geiger Table 12 Main Suppliers of Restriction Enzymes Amersham Buchler (Braunschweig, Germany) Applie ...

Restriction Enzymes 181 probe that hybridizes only to one sequence in a Southern blot experi- ment, one will find in general onl ...

182 Pingoud, Alves, and Geiger 4.5. Cleavage of Single-Stranded DNA The substrate of restriction endonucleases is double-strande ...

Restriction Enzymes 183 clea- lr .- ..... recog ............... hybridization ............ k II II II II II II^11 II II II It II ...

184 Pingoud, Alves, and Geiger Preparative Applications For a variety of different purposes, in particular for the cloning of D ...

Restriction Enzymes 185 removal of single restriction mis-match site I primer aa change.. change of restriction site 2 Hybridiz ...

186 Pingoud, Alves, and Geiger recognition model, the new one is highly redundant, a result that had emerged already from previo ...

Restriction Enzymes 187 bond, polarization of the P-O bond by Mg 2÷ and protonation of the leaving group by a Mg 2÷ bound water ...

188 Pingoud, Alves, and Geiger Kauc, L. and Piekarowicz, A. (1978) Purification and properties of a new restriction endonucleas ...

Restriction Enzymes 189 Pirrotta, V. and Bickle, T. A. (1980) General purification schemes for re- striction endonucleases. Met ...

190 Pingoud, Alves, and Geiger Schoner, B., Kelly, S., and Smith, H. O. (1983) The nucleotide sequence of the HhalI restriction ...

Restriction Enzymes 191 Chandrasegaran, S., Smith, H. O., Amzel, M. L., and Ysern, X. (1986) Pre- liminary X-ray diffraction an ...