Methods in Molecular Biology • 16 Enzymes of Molecular Biology

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Restriction Enzymes 163 note added in proof at end of chapter). Aconcentration of 5-10 mM MgC12 seems to be optimal. Some enzyme ...

164 Pingoud, Alves, and Geiger very pure enzyme preparations are needed for DNA cloning, digestion of genomic DNA for Southern a ...

Restriction Enzymes 165 be detected (182). The radioactive products of these side reactions can be quantitatively determined by ...

166 Pingoud, Alves, and Geiger enzymes. Substrate impurities vary with the method used to isolate and purify the DNA. In particu ...

Restriction Enzymes 167 tion (e.g., BamHI, BstNI). Another class of restriction enzymes (e.g., CfuI, DpnI) only cleaves the sequ ...

168 Pingoud, Alves, and Geiger -5 0 °~ eD 0 0 £9 :=.^0 .=. © N o 5~ % Z ~ ~ uuu~uuuuuu oo ~ oO 0 0 0 0 0 0 09 0 ...

Restriction Enzymes 169 < L3 < L3 (..; < < Z c.~ c e., o ~ ~ ~ .... °~ ee O0 O0 + < r,.3 ~L3 ,~ k~ ~ k3 L3 L3 k3k ...

170 Pingoud, Alves, and Geiger ~Z L) o % z (.9 ~ lao~ ~.~ .-~ =~ ~ ~'-~ ~uuu oe rs u c~e o o o o o o t-- t-- t~ ,~ u~ = ~z (^0 ...

Restriction Enzymes 171 ~Z~ u~ ~ ~ c~ (^0) oo (^0 0) 0 "0 N ...