Methods in Molecular Biology • 16 Enzymes of Molecular Biology

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Restriction Enzymes 193 Kaddurah-Daouk R., Che, P., and Smith, H. O. (1985) Catalytic properties of the HhalI restriction endon ...

194 Pingoud, Alves, and Geiger Armstrong, K. and Bauer, W. K (1982) Preferential site-dependent cleavage by restriction endonuc ...

Restriction Enzymes 195 Mazzarelli, J., Scholtissek, S., and McLaughlin, L.W. (1989) Effects of func- tional group changes in t ...

196 Pingoud, Alves, and Geiger Goodman, H. M., Greene, P. J., Garfin, D. E., and Boyer, H. W. (1977) DNA site recognition by th ...

Restriction Enzymes 197 Yoo, O. J. and Argarwal, K. L. (1980) Isolation and characterization of two proteins possessing HpalI m ...

198 Pingoud, Alves, and Geiger Nilsson, M.-G., Skarped, C., and Magnusson, G. (1982) Structure at restric- tion endonuclease Mb ...

Restriction Enzymes 199 Botstein, D., White, R. L., Skolnik, M., and Davis, R. (1980) Construction of a genetic linkage map in ...

200 Pingoud, Alves, and Geiger Thielking, V., Selent, U., Koehler, E., Wolfes, H., Pieper, U., Geiger, R., Urbanke, C., Winkler ...

CHAPTER 9 DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73) David P. Hornby Introduction DNA methyltransferases (Mtases) cat ...

202 Hornby NH2 NH2 0 ENZ 0 I ENZYME I R R (a) (b) 8AM NH2 R Icl NH2 R Id) Fig. 1. Proposed mechanism of action of 5' cytosine-sp ...

DNA Methyltransferases 203 residue is found in all of the pyrimidine-specific Mtases, which modify at a carbon atom so far chara ...

204 Hornby substrate can be monitored by subsequent restriction enzyme analysis. In each case, the DNA substrate should be caref ...

DNA Methyltransferases 205 responding restriction endonuclease in the case ofprokaryote DNA Mtases. Mammalian dC Mtases have bee ...

206 Hornby catalyzed transfer oftritiated methyl groups to DNA from [3H-methyl] SAM followed by separation of the reaction compo ...

DNA Methyltransferases 207 3.1. Construction of Gene Libraries A partial restriction digest of genomic DNA from any organism can ...

208 Hornby pBR322 ~4~~ plaemld Hi:t/~lb:te d CUT Hlncll SlteHIncll(s) ~ Cleaved Llneerlsed Plasmld Two DNA fragments Fig. 2. Sel ...

DNA Methyltransferases 209 BamHl(a) BamHl(b) //~ ~ Mspl Mtase CUT(a) ~BamHI ~ Plasmldlb) DNA (s~~ lal Two DNA fragments Llnearls ...

210 Hornby Notes Methylated DNA does not transform as efficiently as unmodified DNA into a number of common strains of E. coli. ...

DNA Methyltransferases 211 Lauster, R.,Trautner, T. A., and Noyer-Weidner, M. (1989) Cytosine-specific Type II DNA methyltransf ...