Science - USA (2022-01-21)
showninacryo–electron microscopy (cryo-EM) structure of the SARS-CoV-2 spike protein con- taining the N501YRBDsubstitution bound ...
been noted in other reports when human cells overexpressing ACE2, as opposed to African greenmonkey(Vero)targetcells,wereusedto ...
when it comes to resistance to polyclonal antibodies. One-quarter of sampled individuals had no detectable activity against the ...
A6 activity against infectious SARS-CoV-2 in a plaque reduction neutralization assay (fig. S4B). A3 neutralized almost all SARS- ...
surface areas using molecular dynamic trajec- tories of a fully glycosylated SARS-CoV-2 spike protein with a modeled N370RBDglyc ...
virus spike protein contains a threonine at RBD position 372, which would allow for N370RBDglycosylation (Fig. 5F). Despite the ...
W. F. Garcia-Beltranet al., Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity. Cell 184 , ...
(S10 RR029205). This research used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science Us ...
RESEARCH ARTICLE SUMMARY ◥ PROTEIN DESIGN Reconfigurable asymmetric protein assemblies through implicit negative design Danny D. ...
RESEARCH ARTICLE ◥ PROTEIN DESIGN Reconfigurable asymmetric protein assemblies through implicit negative design Danny D. Sahtoe1 ...
enable rigid fusion at both the N and C termini (fig. S1). Heterodimers with pairedbstrands across the interface were generated ...
designed helical repeat proteins (DHRs) to ter- minal helices ( 23 , 28 ). Because these DHRs have different shapes, they also s ...
Unlike previously designed heterodimers, binding reactions equilibrated rapidly, with affinities ranging from micromolar to low ...
simultaneously and conceptually resemble Ste5 and related scaffolding proteins that organize mitogen-activated protein (MAP) kin ...
nsEM confirmed that this higher-order com- plex is similar to the designed C4-symmetric ring (Fig. 4D and fig. S21). To determin ...
disassembly of the trimer and formation of the ring (Fig. 5B and fig. S23, A to C). Because ring formation is cooperative owing ...
subunit exchange and complex reconfigura- tion in response to signal inputs for synthetic biology and other applications. Becaus ...
Plasmids encoding the individual protomers were ordered in pET29b with an N-terminal polyhistidine His6× tag followed by a TEV c ...
connector and 7.5mM of each terminal cap. (Lower absolute concentrations with the same ratios were used for some trimers.) ABCA ...
with substrate, and immediately recorded. All kinetic measurements were fitted to a single exponential decay function: S=A*exp(− ...
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