Science - USA (2022-02-11)

some beneficial effects of caloric restriction. —LBR Science, abg7292, this issue p. 671; see also abn6576, p. 620 CORONAVIRUS V ...

from the tissue pixels containing single nuclei. Spatial-CUT&Tag adds a new dimension to spatial biology by enabling the map ...

GWAS analysis solely for coffee and tea preferences, which, however, identified specific genetic variants associated with caffei ...

627-B 11 FEBRUARY 2022 • VOL 375 ISSUE 6581 science.org SCIENCE RESEARCH CELL BIOLOGY The making and removal of a leader When ep ...

SCIENCE science.org 11 FEBRUARY 2022 • VOL 375 ISSUE 6581 627-C RESEARCH selecting for dosage compensa- tion and sexually antago ...

RESEARCH ARTICLE SUMMARY ◥ CELL BIOLOGY p53 directs leader cell behavior, migration, and clearance during epithelial repair Kasi ...

RESEARCH ARTICLE ◥ CELL BIOLOGY p53 directs leader cell behavior, migration, and clearance during epithelial repair Kasia Kozyrs ...

p21 promotes the emergence of leader cells downstream of p53 Spontaneous leader cells, BBCs, and MMC- treated cells are all cell ...

(fig. S4, E to J). Induction of leaders by p21 overexpression was also sufficient to up-regulate PI3K and ITGb1(fig.S4,OtoR),whi ...

under the control of p21 promoter sequences that respond to p53 (fig. S6J) ( 30 ). We found that scratching induced p53 activity ...

with high p53 (Fig. 6E), neither did it rescue their loser status in competition experiments with p53 mutant cells (Fig. 6F and ...

Cell culture and plasmids MDCK cells were maintained in Dulbecco’s minimum essential medium (21885, Thermo Fisher Scientific) su ...

used: 5′-CGGCAAGCCTTGCTGCCATG(AGG)- 3 ′and 5′-TGGACAGCGAGCAGCTGCGC(CGG)- 3 ′. sgRNAs were cloned individually into PX461 vectors ...

(PCR) product was introduced into doxycycline- inducible pTRIPZ-RFP-NLS-Puro ( 24 ) with a P2A peptide bridging the two proteins ...

Real-time quantitative reverse-transcription PCR (RT-qPCR) On day 1, 400,000 MDCK cells per well were seeded in a six-well plate ...

The migration speed of scratched epithelial fronts was measured in FIJI by manually drawing freehand lines outlining the wound e ...

RESEARCH ARTICLE SUMMARY ◥ IMMUNOLOGY CD97 promotes spleen dendritic cell homeostasis through the mechanosensing of red blood ce ...

RESEARCH ARTICLE ◥ IMMUNOLOGY CD97 promotes spleen dendritic cell homeostasis through the mechanosensing of red blood cells Dan ...

Liuet al.,Science 375 , eabi5965 (2022) 11 February 2022 2 of 13 A DCIR2 CD8 12.6 74.0 25.2 46.4 19.7 41.8 43.8 28.5 Control Gna ...

contains the GPCR proteolysis site (GPS). The N-terminal fragment (NTF) of CD97 remains noncovalently bound to the seven-transme ...