Science - USA (2022-02-11)

cDC2s in blood circulation (fig. S2, K and L). Sampling of blood in the splenic artery and vein revealed that cDC2s were most in ...

Liuet al.,Science 375 , eabi5965 (2022) 11 February 2022 5 of 13 A 80 60 40 20 0 100 Saline *** 1.5 1.0 0.5 0 2.0 Saline *** B 8 ...

GSEA showed that Mrtf-a–dependent genes ( 29 ) were up-regulated in ArhGEF1- and CD97- deficient cDC2s (Fig. 5E). Many of the Mr ...

cDC2s (Fig. 6A and fig. S7A). In accord with the reduced antigen capture, CD97-deleted cDC2s showed less efficient activation as ...

reactions, potentially resulting in hemolytic dis- eases and renal failure ( 7 , 34 ). Using transfers of foreign antigen conjug ...

further test the contribution of reduced CD97 expression to theIrf4cKOphenotype, we selectively restored CD97 expression in IRF4 ...

cells up-regulate their F-actin, and Mrtf tran- scription factors become active and drive ex- pression of many cytoskeleton-asso ...

BV711-conjugated anti-CD4 (GK1.5), PE-conjugated anti-CD278 (15F9), PE/Cy7-conjugated anti-CD95 (SA367H8), Pacific Blue-conjugat ...

Cell count was analyzed with Imaris automatic spot tracking aided by manual verification, and cell migration was analyzed and ve ...

imaging analysis, J. Muppidi and X. Piao for helpful discussions, and N. Carey for advice on statistical analysis.Funding:D.L. w ...

Where Science Gets Social. AAAS’ MemberCommunityisaone-stopdestinationforscientists and STEM enthusiasts alike. It’s“WhereScienc ...

RESEARCH ARTICLE SUMMARY ◥ IMMUNOLOGY Marginal zone B cells acquire dendritic cell functions by trogocytosis Patrick Schriek, Al ...

RESEARCH ARTICLE ◥ IMMUNOLOGY Marginal zone B cells acquire dendritic cell functions by trogocytosis Patrick Schriek^1 , Alan C. ...

transfer was observed from B cells to cDCs (fig. S3D). MZ B cells were more trogocytic than their follicular (FO) counterparts ( ...

Schrieket al.,Science 375 , eabf7470 (2022) 11 February 2022 3 of 12 Fig. 2. MARCH1- deficient mice harbor MZ B cells expressing ...

cDCs (Fig. 5B), even though MARCH1 is functional in these cells as shown by their wild-type expression of CD86 (fig. S4F). To co ...

binding to MHC II has been conserved through evolution. CR2 drives B cell trogocytosis of cDC membranes containing MHC IIÐC3 com ...

MZ B cell trogocytosis occurs constitutively in wild-type mice. Finally, we tested whether trogocytosis enabled MZ B cells to“hi ...

first is cellular and features cDCs and MZ B cells. ThemainroleofcDCsistopresentAgtoTcells andinitiateadaptiveimmunity( 18 ). In ...

enriched by density gradient centrifugation (at 2237g) with 1.077 g/cm^3 Nycodenz (Axis shield) and subsequent negative depletio ...