Science - USA (2022-04-08)

was cloned into pAcGP67a vector with acid zipper. 2mL baculovirus linear DNA and 2mg TCR constructs were mixed with 100mL Opti- ...

T cells, the chip was heated to and main- tained at 37°C for 1 min and then cooled to and kept at 34°C for 2 min. Immediately af ...

S. T. Kimet al., TheabT cell receptor is an anisotropic mechanosensor.J. Biol. Chem. 284 , 31028–31037 (2009). doi:10.1074/jbc. ...

RESEARCH ARTICLES ◥ VIROME Cryptic and abundant marine viruses at the evolutionary origins of EarthÕs RNA virome Ahmed A. Zayed1 ...

SCIENCEscience.org 8 APRIL 2022¥VOL 376 ISSUE 6589 157 Fig. 1. Establishment of RdRp domain megaclusters.(A) Arctic projection o ...

was groundbreaking, RdRp phylogenies are complex and require a manual and stepwise approach for construction, including a labo- ...

suggested names are provided in the supple- mentary materials, materials and methods). In the current orthornaviran megataxonomi ...

strongly monophyletic [Duplornaviricotaand Negarnaviricotaare labeled as branches 4 and 5, respectively, in ( 7 , 17 )]. However ...

genome types (+ssRNA,−ssRNA, and dsRNA viruses) have multiple evolutionary origins. Third, we revisited the RdRp primary sequenc ...

“Taraviricota”and“Arctiviricota”are likely mostly derived from their genomes (fig. S8). Together, these data provide an orthorna ...

STRUCTURAL BIOLOGY Structure of a Janus kinase cytokine receptor complex reveals the basis for dimeric activation Caleb R. Glass ...

164 8 APRIL 2022¥VOL 376 ISSUE 6589 science.orgSCIENCE Fig. 1. Purification and biochemical characterization of an active JAK1-I ...

in which the zippers approximated the spatial constraints of dimerized TM domains (Fig. 1B) ( 36 , 37 ). Coexpression of the sol ...

ICDs as they would extend from the TM re- gions mimicked by the GCN4 zippers. The FERM-SH2 modules sit above inward-facing PK do ...

understand the structural influence of the V→F mutation, we modeled the WT Val^657 into the structure. The smaller Val side chai ...

monomer suggests that it adopts a dynamic range of conformational states from a com- pact“closed”state to an extended“open”state ...

models for the PK-TK domains that form head-to-head dimers through interactions between kinase N lobes, which could poten- tiall ...

PARTICLE PHYSICS High-precision measurement of theWboson mass with the CDF II detector CDF Collaboration à, T. Aaltonen1,2, S. A ...

references therein]. Many of these hypotheses include a source of dark matter, which is cur- rently believed to comprise ~84% of ...

energy of 1.96 TeV. The (anti)quark momen- tum distributions in the (anti)proton are the best-measured among all constituent par ...