Science - USA (2022-04-08)

conventional and plasmacytoid dendritic cells (cDC and pDC); CD4+and CD8+T cells (CD4 and CD8); natural killer cells (NK); B cel ...

Perezet al.,Science 376 , eabf1970 (2022) 8 April 2022 3 of 13 cDC pDC cM ncM NK Lymphoid B Myeloid Prolif CD4 UMAP1 UMAP2 CD8 P ...

Perezet al.,Science 376 , eabf1970 (2022) 8 April 2022 4 of 13 A CD4Naive CD8Naive CD4EM CD4Reg CD8GZMH CD8GZMK CD8MAIT NKBright ...

to the high correlation between signature genes calculated across CD8GZMHpseudobulk ex- pression profiles from different individ ...

analysis (PCA) of expression features followed by K-means clustering to identify two clusters that broadly separated donors by c ...

Perezet al.,Science 376 , eabf1970 (2022) 8 April 2022 7 of 13 AB Thrombocytopenia Renal involvement Tup Bup anti-Smith SLADAI L ...

These results show that cell type–specific ex- pression profiles obtained using mux-seq can be used to link cell-intrinsic state ...

that reveal the region overlapping rs11080327 to be harboring a cis-regulatory element that is activated in response to type 1 i ...

states in SLE. Compositionally, the decrease of naïve CD4+T cells in cases, particularly those of Asian ancestry, appears to exp ...

CD8+T and NKdimcells. AlthoughGZMBand PRF1have been described as markers for CD8+ T cell subsets enriched in SLE ( 54 ),GZMHwas ...

The 10x Chromium scATAC-seq kit was used to process PBMCs from five healthy individu- als incubated for 8 hours with IFNB or cul ...

R. K. Perezetal., Multiplexed scRNA-seq reveals the cellular and genetic correlates of systemic lupus erythematosus Analysis Co ...

GOLDOPENACCESS,DIGITAL,ANDFREETO ALLREADERS AsAAAS’s firstmultidisciplinary, openaccessjournal,ScienceAdvancespublishes research ...

RESEARCH ARTICLE SUMMARY ◥ IMMUNOGENOMICS Single-cell eQTL mapping identifies cell typeÐspecific genetic control of autoimmune d ...

RESEARCH ARTICLE ◥ IMMUNOGENOMICS Single-cell eQTL mapping identifies cell typeÐspecific genetic control of autoimmune disease S ...

Yazaret al.,Science 376 , eabf3041 (2022) 8 April 2022 2 of 14 UMAP 1 UMAP 2 CD14 0.23 CD4 0.05 CD8a 0.07 SLC4A10 0.4 CCR7 0.05 ...

single-cell sequencing studies ( 26 , 29 , 30 ) (Fig. 1H). After batch correction, we found no evidence for variation in cell id ...

Yazaret al.,Science 376 , eabf3041 (2022) 8 April 2022 4of14 024 6 Total eQTLs (10^6 ) 20 40 Independent eQTLs (10 2 ) 0.75 1.0 ...

that the eQTLs result from independent var- iants that influence expression in different cell types. To test between these hypot ...

diseases ( 45 Ð 48 ) and have a dynamic eQTL ef- fect onORMDL3in B cells across the trajectory. Genetic variation controls trans ...