Cell - 8 September 2016
IGHV1-202. Thus, we generated a related, but distinct, mouse model to facilitate VRC01 affinity maturation studies by inte- grat ...
IgL-expressing B cells that could serve as potential targets for immunization. Induction of Neutralizing Antibodies through Step ...
(legend on next page) 1478 Cell 166 , 1471–1484, September 8, 2016 ...
VRC01 antibody, were mutated and the variable domains 1, 2, and 3 were deleted (McGuire et al., 2016). Although eOD-GT6 60-mer a ...
toward three different forms of engineered gp120 outer domain or cores that interact with germline VRC01-class antibodies (eOD-G ...
Figure 7. Selected IGHV1-202/IGKV3-2001 IgGs Cloned from the Stepwise Immunized Mice Neutralized the Same Viruses Sensitive to t ...
immunization with a high-affinity germline VRC01 binder acti- vated B cells that express IGHV1-202 in association with a mouse I ...
that are compatible with normal B cell maturation. It will be of in- terest to apply our de novo recombination strategy to the e ...
Hu, J., Meyers, R.M., Dong, J., Panchakshari, R.A., Alt, F.W., and Frock, R.L. (2016). Detecting DNA double-stranded breaks in m ...
STAR+METHODS KEY RESOURCES TABLE REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 3181_F12_H This paper KX379632 3181_E7_H This ...
Continued REAGENT or RESOURCE SOURCE IDENTIFIER 1539-G6_H This paper KX462805 1538-1_H This paper KX462806 1538-4_H This paper K ...
Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins C13.G3 This paper KX462845 426c-de ...
CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for reagents may be directed to, and will be fulfilled ...
flanked with homology arms for the VH81X locus. The 5^0 homology arm is 3.2kb long and corresponds to the sequences upstream of ...
that the final reaction contains: 1xCoral Red PCR buffer, 0.5 units of Taq polymerase, 0.2mM dNTP, 10pmol of 5^0 IGKV3-2001 prim ...
ELISA Corning Costar half-area assay plates were coated with antigens at 100 ng/well at 4C overnight, blocked with either B3T b ...
mutations, and those with frequency lower than 95% CI were counted as depleted mutations. Sequences were aligned using MUSCLE (E ...
Supplemental Figures (legend on next page) ...
Figure S1. Generation and Characterization of the VH1-2 Mouse Model, Related toFigures 1and 3 (A and B) Results of Southern anal ...
(legend on next page) ...
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