Cell - 8 September 2016
N276 glycan site may simply be a normal feature of partially mature VRC01-class antibodies (Jardine et al., 2016b; Kong et al., ...
Burton, D.R., and Hangartner, L. (2016). Broadly neutralizing antibodies to HIV and their role in vaccine design. Annu. Rev. Imm ...
Pancera, M., McLellan, J.S., Wu, X., Zhu, J., Changela, A., Schmidt, S.D., Yang, Y., Zhou, T., Phogat, S., Mascola, J.R., and Kw ...
STAR+METHODS KEY RESOURCES TABLE CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for reagents may be d ...
EXPERIMENTAL MODEL AND SUBJECT DETAILS Mice All animal studies were approved by The Scripps Research Institute Institutional Ani ...
Single-Cell Sorting by Flow Cytometry Mice spleen and lymph node samples were processed for single B cell sorting based on previ ...
72 C for 2 min; 72C for 7 min. RT-PCR reactions were purified using 0.8 volumes of SPRIselect magnetic beads (Beckman- Coulter ...
calculations were performed in Graphpad Prism. The number of replicates, the type of data represented in figure plots, and a des ...
Supplemental Figures Figure S1. Development of Boosting Immunogen GT3.1, Related toFigure 1 Sequence alignment of BG505 SOSIP.66 ...
Figure S2. Sequence and Characterization of BG505 Core-GT3.1 Nanoparticles, Related toFigure 1 (A) Sequences of the two genes co ...
Figure S3. Characterization and Structural Analysis of BG505 GT3.1 SOSIP and ABC SOSIP Trimers, Related toFigure 1 (A) Negative- ...
Figure S4. Sequence and Antigenicity of the r1-Core Resurfaced Core gp120, Related toFigure 2 (A) Sequence alignment of r1-core ...
Figure S5. Serum Binding to r1-Core and r1-Core-KO, Related toFigure 2 Serum binding to r1-core (red, also referred to as HXcore ...
Figure S6. Broad Neutralization of Near-Native Viruses by VRC01-gH mAbs, Related toFigure 5 Neutralization of a 25-virus panel c ...
Article Induction of HIV Neutralizing Antibody Lineages in Mice with Diverse Precursor Repertoires Graphical Abstract Highlights ...
Article Induction of HIV Neutralizing Antibody Lineages in Mice with Diverse Precursor Repertoires Ming Tian,1,2,11Cheng Cheng,3 ...
gene segments. VRC01 IgL chain CDR3s (CDR L3s) are invari- ably 5-amino acid (aa) long, which is far below the average hu- man C ...
control mice (Figure S1C). To assess frequency of IGHV1-202 and D usage, as well as CDR3 length and diversity among peripheral B ...
A B C DEF GH Figure 2. Elicitation of VRC01-Class Germline Antibodies in VH1-2 Mouse Model (A) Immunization schema. (B) Sera bin ...
ability of candidate immunogens to selectively prime B cells ex- pressing antibodies with VRC01 signatures. Selective Activation ...
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