RNA Detection

3.4 Preclearing of Lysate On the day of the experiment, warm frozen aliquots of either whole cell lysate or nuclear lysate to 3 ...

Wash beads 3–6 times, with at least one bead volume of 1 Hybridization Buffer per wash. Incubate each wash for 5 min at 67 C. ...

Resuspend beads in 60μL RLT Buffer (3original sample volume). Transfer beads in RLT to 20μL RNA sample and mix well. Add 120μL ...

Digest samples with 0.1μg Lysyl endopeptidase for 4 h at room temperature. Dilute samples to final concentration of 2 M urea by ...

avoiding multiple freeze–thaw cycles. Sequences for control capture oligonucleotide probes for mouse RNA targets (U1, 18S, and 4 ...

medium for at least three generations and the incorporation should be tested by mass spectrometry to ensure>95% labeling of p ...

the Input plus Probe sample during RT-qPCR controls for any DNA probe that was not removed during RNA cleanup. 10.Cleaning of pe ...

Acknowledgments We thank Christine Surka, Alexander Shishkin, and Jesse Engreitz for theirhelp in developingthe RAP-MS method an ...

INDEX A Affinity purifications....................................49–57, 85–93, 405–407, 410, 412, 414, 420, 423 Alkaline fragme ...

G Gel electrophoresis agarose gel electrophoresis.........................32, 38, 53, 92, 187, 189, 194, 456, 460 2D PAGE gel el ...

O 2’-O-methyl RNA.......................................233, 244, 245 Oocytes................................................... ...

Transcription................................................ 4, 31, 70, 79, 82, 98, 115, 141, 144, 210, 267, 273, 295, 307, 349 ...