Synthetic Biology Parts, Devices and Applications

16.3 Generation of Pathway Libraries 335 of the pathway one at a time to increase flux to the desired product [25–27], but pathw ...

Gibson assembly enzyme and promoter library DNA assembler promoter library DNA assembler enzyme library Enzyme error-prone libra ...

16.3 Generation of Pathway Libraries 337 reporter genes mKate2 and sfGfp. The promoter library was tested using this fluorescent ...

338 16 Applying Advanced DNA Assembly Methods to Generate Pathway Libraries was used as a proof-of-concept study for this assemb ...

16.3 Generation of Pathway Libraries 339 16.3.2 In vivo Assembly Methods E. coli does not have robust and efficient homologous r ...

340 16 Applying Advanced DNA Assembly Methods to Generate Pathway Libraries 16.3.2.2 In vivo Plasmid Assembly and One-Step Optim ...

16.3 Generation of Pathway Libraries 341 least a 400-bp region homologous to the 5′ and 3′ DNA regions of the pathway at the ter ...

0102030405060 0 5 10 15 20 25 30 35 40 Time (h) (a) (b) (c) (d) 01020304050 50100150200250 50100150200250 60 0 20 40 60 80 100 T ...

16.4 Conclusions and Prospects 343 separately (Figure 16.2). The total library size screened was 10^4 and was screened for strai ...

344 16 Applying Advanced DNA Assembly Methods to Generate Pathway Libraries Pathway libraries A collection of mutant enzymatic p ...

References 345 14 Shetty, R.P., Endy, D., and Knight, T.F. Jr. (2008) Engineering BioBrick vectors from BioBrick parts. J. Biol. ...

346 16 Applying Advanced DNA Assembly Methods to Generate Pathway Libraries 30 Ramon, A. and Smith, H.O. (2011) Single-step link ...

References 347 require moderate D-xylulokinase activity. Appl. Environ. Microbiol., 69 , 495–503. 45 Matsushika, A., Watanabe, S ...

349 Synthetic Biology: Parts, Devices and Applications, First Edition. Edited by Christina Smolke. © 2018 Wiley-VCH Verlag GmbH ...

350 17 Synthetic Biology in Immunotherapy and Stem Cell Therapy Engineering instabilities that enable them to escape individual ...

17.3 Synthetic Biology Approaches to Cellular Immunotherapy Engineering 351 synapse [30, 31] or stably integrating T cells with ...

352 17 Synthetic Biology in Immunotherapy and Stem Cell Therapy Engineering characteristic and significant advantage of cellular ...

17.3 Synthetic Biology Approaches to Cellular Immunotherapy Engineering 353 activity toward the target cell. Second- and third- ...

354 17 Synthetic Biology in Immunotherapy and Stem Cell Therapy Engineering ζ ζ ζ ζ T-cell membrane Antigen-binding domain (e.g. ...

17.3 Synthetic Biology Approaches to Cellular Immunotherapy Engineering 355 sequential antigen encounter. Such computational cap ...