Microfluidics for Biologists Fundamentals and Applications

environments rather than in laboratory conditions. Lack of funds may be another reason for the slow pace of transformation of ac ...

References Mark D, Haeberle S, Roth G, von Stetten F, Zengerle R (2010) Microfluidic lab-on-a-chip platforms: requirements, cha ...

Kang L, Chung BG, Langer R, Khademhosseini A (2008) Microfluidics for drug discovery and development: from target selection to ...

Kim J-Y, Fluri DA, Marchan R, Boonen K, Mohanty S, Singh P, Hammad S, Landuyt B, Hengstler JG, Kelm JM, Hierlemann A, Frey O (2 ...

McClain MA, Culbertson CT, Jacobson SC, Allbritton NL, Sims CE, Ramsey JM (2003) Microfluidic devices for the high-throughput c ...

Legendre LA, Morris CJ, Bienvenue JM, Barron A, McClure R, Landers JP (2008) Toward a simplified microfluidic device for ultra- ...

Chapter 9 On-Chip Immunoassay for Molecular Analysis Andy Ng 1 Introduction 1.1 Immunoassays The specificity and affinity of ant ...

antibody complexes are detected, or an increase in signal. In non-competitive mode, there is an excess of antibodies. The antige ...

2.1 Homogeneous Immunoassays in Microfluidic Systems Homogeneous microfluidic immunoassays rely on the discrimination of analyte ...

simultaneous direct immunoassays for ovalbumin and anti-estradiol using fluores- cence detection, completing the assay within 1 ...

functional carboxyl groups, which form electrostatic interactions with biotin-PLL- g-PEG. NeutrAvidin labeled-Protein A binds st ...

which the flow of the sample is joined perpendicularly by a confinement makeup flow of buffer. Since the flow is laminar, the ma ...

A unique magnetic bead retention mechanism has been developed by Lacharme et al., [ 46 ] which exploited the self-assembling pro ...

with the capture antibody. Using mouse IgG as a model analyte, this method reached a detection limit of 250 pg/mL when operating ...

network to adsorb the analyte onto a PDMS-coated glass slide. Another PDMS bearing H-shaped microchannels for reagent delivery w ...

3.2 Pressure-Driven Fluid Handling Modalities The most popular fluid handling modality for microfluidic immunoassay is by pressu ...

For more precise control of pressure-driven flow and automation, micropneumatic pumps integrated with the microfluidic chip have ...

chambers that perform many analytical functions, including sample/reagent trans- port, dilution, mixing and separation, all with ...

3.4 Passive, Capillary Force-Driven Modalities Passive fluid handling has been popular because the integration of active actuati ...

flow, which then automatically triggered the fluidic operations to complete the immunoassay. However, the system was unable to s ...