Science - USA (2022-06-03)
RESEARCH ARTICLE SUMMARY ◥ MICROBIOLOGY High-throughput, single-microbe genomics with strain resolution, applied to a human gut ...
RESEARCH ARTICLE ◥ MICROBIOLOGY High-throughput, single-microbe genomics with strain resolution, applied to a human gut microbio ...
We introduce Microbe-seq, a high-throughput method for obtaining the genomes of large numbers of individual microbes. We use mi- ...
itsconstituentreadslikelyconnectwithamix of different closest-aligned genomes; by con- trast, if the reads from a SAG originate ...
each coassembled genome against a public database (GTDB-Tk) ( 59 ), using the ANI >95% criterion to identify matches of the s ...
complex microbial communities and express different sets of genes to carry out these roles ( 62 ). Linking specific genes and co ...
highest number of SAGs, all SAGs in each cluster share fewer than 10% of the bases at SNP locations with the consensus genotype ...
Notably, we find several genes that occur in the HGT sequences of six or seven Bacteroi- detes strains. We examine the HGT seque ...
have not been cultured. This high-throughput microfluidics-based approach allows for more practical individual examination of a ...
The incubation program for MDA is: 30°C for 6-8 hours, 65°C for 10 min and sample storage at 4°C. Tagmentation We merge sample d ...
its reads to the most-aligned reference genome to determine its genome coverage. Genome coassembly of microbial species in the h ...
location, and SNPs with fewer than two SAGs being the reference allele or fewer than two SAGs being the mutation allele. We also ...
I. Cho, M. J. Blaser, The human microbiome: At the interface of health and disease.Nat. Rev. Genet. 13 , 260–270 (2012). doi:10 ...
R. Ding, W. L. Ung, J. A. Heyman, D. A. Weitz, Sensitive and predictable separation of microfluidic droplets by size using in-l ...
RESEARCH ARTICLE SUMMARY ◥ IMMUNOLOGY Mapping the developing human immune system across organs Chenqu Suo†, Emma Dann†, Issac Go ...
RESEARCH ARTICLE ◥ IMMUNOLOGY Mapping the developing human immune system across organs Chenqu Suo1,2†, Emma Dann^1 †,IssacGoh^3 ...
thymus, and spleen from two donors at 16 and 18 pcw with spatial transcriptomics (10X Ge- nomics Visium Spatial Gene Expression) ...
localized to the thymic cortex, whereas mature T cells were consistently mapped to the thymic medulla. Furthermore, in two of th ...
T cells could be subdivided into a subset en- riched in the thymus and other subsets en- riched in peripheral organs (Fig. 3C). ...
NF-kB signaling accompanying ABT(entry) to thymic mature T cells, expression of NF-kB- signaling genes continued to increase whe ...
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