Nature - 15.08.2019
reSeArCH Letter Extended Data Fig. 2 | SidJ binds to CaM. a, GFP and GFP–SidJ were ectopically expressed in HEK293T cells, and i ...
Letter reSeArCH a) b) 0500 1000 1500 2000 0 10000 20000 30000 40000 Time [s] Fluorescence (AU ) SdeA SdeA + SidJ SdeA + SidJ + C ...
reSeArCH Letter c) 100 80 Relative intensity (%) 0 40 60 20 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 30 ...
Letter reSeArCH -QVGRHGEGTESEFSVYLPEDVALVPVK- Glu Glu -QVGRHGEGTESEFSVYLPEDVALVPVK- Glu Glu -QVGRHGEGTESEFSVYLPEDVALVPVK- Di-Glu ...
reSeArCH Letter a) b) e) f) c) Marker kDa^13 18 19 2017161514 21 22 23 2425 26 80 32 25 17 11 (^00102030) 200 400 600 Volume (ml ...
Letter reSeArCH 741.854 Particles 1.506.073 Particles picked (WARP) 2 Rounds of 2D classication with 2-fold binned particles (1 ...
reSeArCH Letter 5.1 (Å) 4.8 4.6 4.3 3.9 b) c) d) a) SidJ Human apo CaM b) Resolution (1/Å) Fourier shell correlation (FSC ) . SU ...
Letter reSeArCH Canonical pocket with PPi Migrated pocket with AMP SidJ CaM a) b) c) EM map showing electron density in the migr ...
reSeArCH Letter Extended Data Fig. 10 | SidJ-dependent glutamylation during Legionella infection. a, Raw264.7 macrophages were i ...
1 nature research | reporting summary October 2018 Corresponding author(s): Sagar Bhogaraju, Ivan Dikic Last updated by author(s ...
2 nature research | reporting summary October 2018 Field-specific reporting Please select the one below that is the best fit for ...
3 nature research | reporting summary October 2018 Ubiquitin Ubi-1 from abcam (Cat:ab7254), validation statement from the manufa ...
Letter https://doi.org/10.1038/s41586-019-1439-1 Regulation of phosphoribosyl ubiquitination by a calmodulin-dependent glutamyla ...
reSeArCH Letter family effectors from L. pneumophila, which ubiquitinate structurally diverse proteins that are associated with ...
Letter reSeArCH 563 to 910, hereafter denoted SdeA(563–910))^11 that was purified from HEK293T cells expressing GFP–SidJ (Flag–m ...
reSeArCH Letter glutamylase that catalyses the ligation of glutamate moieties to E860 of SdeA. We further investigated the mecha ...
Letter reSeArCH against EGTA or upon inclusion of this chelator in the reactions (Extended Data Fig. 6b, c). The presence of AMP ...
reSeArCH Letter Methods Media, bacteria strains, plasmid constructions and cell lines. L. pneumophila strains used in this study ...
Letter reSeArCH resolved by SDS–PAGE and stained with Coomassie Brilliant Blue. Gels were then dried and signals were detected w ...
reSeArCH Letter Extended Data Fig. 1 | Determination of the modification rate of E860 of SdeA. a, Peak areas of the extracted-io ...
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