Nature 2020 01 30 Part.02

c 0102030 0 20 40 60 80 100 Days Percent surviva l IC GFP-mRNA IC PD1 CTLA4 TIM3 IC VEGF-C-mRNA + PD1 CTLA4 TIM3 P = 0.01 P = 0. ...

1 nature research | reporting summary October 2018 Corresponding author(s): Akiko Iwasaki, Jean-Leon Thomas Last updated by auth ...

2 nature research | reporting summary October 2018 Field-specific reporting Please select the one below that is the best fit for ...

3 nature research | reporting summary October 2018 (17A2, biotin, 1:500), anti-CD31 (2H8, GeneTex, 1:500) and anti-LYVE1 (AF2125 ...

4 nature research | reporting summary October 2018 Methodology Sample preparation Isolation of mononuclear cells and flow cytome ...

Nature | Vol 577 | 30 January 2020 | 695 Article Mechanism of adrenergic CaV1.2 stimulation revealed by proximity proteomics Guo ...

696 | Nature | Vol 577 | 30 January 2020 Article GFP-tagged 28-mutant β2B subunits dominate in the 35-mutant α1C complex (Extend ...

Nature | Vol 577 | 30 January 2020 | 697 on the CaV1.2 proteome signature in Langendorff-perfused whole hearts (Fig. 2g, h). We ...

698 | Nature | Vol 577 | 30 January 2020 Article validating the mechanism. We now find that Rad was the missing ingre- dient. To ...

Nature | Vol 577 | 30 January 2020 | 699 We used a flow-cytometry Förster resonance energy transfer (FRET) two-hybrid assay^30 t ...

700 | Nature | Vol 577 | 30 January 2020 Article by PKA phosphorylation is reminiscent of PKA phosphorylation of phospholamban, ...

Methods Clone construction and cell culture All mouse N-terminally GFP-tagged Rad (GenBank accession number XM_006531206) constr ...

Article an anti-calmodulin antibody (Millipore Sigma 05-173; 1/1,000 dilu- tion), a custom-made anti-RyR2 antibody (1/5,000 dilu ...

were then fractionated by alkaline reversed phase chromatography into 96 fractions and combined into 24 samples. Mass-spectromet ...

Article the perforated whole-cell patch-clamp technique in order to minimize current run-down and preserve the intracellular mil ...

For Cer–Ven dimers, we obtained FRET efficiencies of roughly 0.55, 0.38, and 0.05 for linker lengths 5, 32, and 228 respectively ...

Article Extended Data Fig. 1 | Putative PKA phosphorylation sites in α1C and β2B subunits. a, Left column, the 35 putative PK A ...

Extended Data Fig. 2 | Traff icking and function of APEX2-conjugated CaV1.2 subunits in heart. a, Exemplar current–voltage relat ...

Article Extended Data Fig. 3 | Analysis of proteins quantif ied by mass spectrometry in cardiomyocytes isolated from α1C–APEX2 a ...

Extended Data Fig. 4 | See next page for caption. ...