Science - 31 January 2020
represent candidates that may shift toward higher polysome occupancy in response to synaptic stimulation. Furthermore, given the ...
cocktail (Roche)] ( 25 )bydouncinginaglass homogenizer. For the experiments including RNase inhibitors, the polysome lysis buffe ...
(1/5000, Licor); anti-rabbit IR680 (1/5000, Licor)]. Protein levels in bands of interest were quantified using ImageJ (NIH). Wes ...
precursor tolerance for mass recalibration, and the main search mass tolerance was set to 4.5 ppm. The fragment mass tolerance w ...
fraction, a one-way analysis of variance (ANOVA) was performed. A significantPvalue rejects the null hypothesis that all frames ...
T. E. Graberet al., Reactivation of stalled polyribosomes in synaptic plasticity.Proc. Natl. Acad. Sci. U.S.A. 110 , 16205 – 16 ...
M. Martin, Cutadapt removes adapter sequences from high-throughput sequencing reads.EMBnet.journal 17 ,10– 12 (2011). doi:10.14 ...
RESEARCH ARTICLE SUMMARY ◥ CELL BIOLOGY Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles Jas ...
RESEARCH ARTICLE ◥ CELL BIOLOGY Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles Jason E. Le ...
PBs (~40 PBs per cell, Fig. 1D), and this led to an increase in the percentage of PBs that were associated with the ER (80.4 ± 3 ...
cells to shift the balance of cisternae and tu- bules toward more cisternal ER.RTN4knock- out (KO) cells displayed significantly ...
Leeet al.,Science 367 , eaay7108 (2020) 31 January 2020 4of10 Fig. 3. The relationship between ER shape and PB biogenesis. (A) R ...
well-studied translation inhibition and RNP granule biogenesis response ( 17 ). We observed a significant increase in PB and str ...
nascent proteins in the ER lumen. Wild-type andRTN4KO cells were treated with Tm for 1 and 6 hours to resolve any differences be ...
neck, suggesting that the ER might contrib- ute to constriction initiation during the fission process (Fig. 6, E and F; Movie 5; ...
Leeet al.,Science 367 , eaay7108 (2020) 31 January 2020 8of10 ER tubules RNP granule Linescan of fluorescence intensities to ass ...
CA). RA-Sec61bwas generated by amplifying the Sec61b-encoding sequence from GFP-Sec61b and inserting it into XhoI/KpnI sites of ...
Upon identification of PB or stress granule fission during postimaging analysis, a seg- mented line was drawn perpendicular to t ...
RESEARCH ARTICLE ◥ CELLULAR NEUROSCIENCE Microglia monitor and protect neuronal function through specialized somatic purinergic ...
(n= 268 cells from 2 mice). Thus, these contacts are evolutionary conserved and present in all main areas of the brain. Microgli ...
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