Nature - USA (2019-07-18)
Letter reSeArCH Methods Cell culture. HEK 293T/17 (ATCC CRL-11268) cells, U2OS (ATCC HTB-96) cells, and NIH3T3 (ATCC CRL-1658) c ...
reSeArCH Letter were performed approximately 24 h after the initial formation of DF2-enriched droplets. For fluorescent m^6 A-RN ...
Letter reSeArCH preparation was performed using the NEBNext Ultra Directional RNA Library Prep Kit starting from 1 μg of total i ...
reSeArCH Letter Statistics and reproducibility. All statistical analyses were performed in GraphPad Prism 8 or Microsoft Excel u ...
Letter reSeArCH c YTH YTHDF2 YTH YTHDF2 Buffer edge Buffer edge 1 μg100 ng10 ng1 ng 0 m^6 A RNA 10 m^6 A RNA 1 m^6 A RNA Methyle ...
reSeArCH Letter Extended Data Fig. 1 | Fluorescent labelling of DF2 does not affect the formation of liquid droplets. a, DF1, DF ...
Letter reSeArCH a b c G3BP1 G3BP1 Merge Merge Arsenite (1 h) Heat Shock (30 min) d DF1 Merge DF3 Merge Heat Shock (30 min)Heat S ...
reSeArCH Letter Extended Data Fig. 2 | Assessing which stressors induce stress granules and the localization of DF2 proteins in ...
Letter reSeArCH Extended Data Fig. 3 | Confirmation of the Mettl14-knockout model and DF2 phase-separation into P-bodies in mES ...
reSeArCH Letter a No stress HS Ars YTHDF2 G3BP1 GAPDH Tubulin SG Marker Cytosol Markers NucCytSGSolNucCytSGSolNucCytSGSol d Cumu ...
Letter reSeArCH Extended Data Fig. 4 | The number of m^6 A nucleotides is correlated with stress-granule enrichment independent ...
reSeArCH Letter Extended Data Fig. 5 | Detection of translation in mES cells after stress. a, m^6 A-mRNA transcript abundance is ...
Letter reSeArCH -log10 (p-value) Fold Enrichment (m 6 A mRNA/non-m 6 A mRNA) Biological Process Molecular Function Cellular Comp ...
reSeArCH Letter Extended Data Fig. 6 | Gene ontology of polymethylated and singly methylated mRNAs in U2OS cells. a, b, Gene ont ...
Letter reSeArCH Extended Data Fig. 7 | Model of how the properties of m^6 A-containing mRNAs are determined by their phase separ ...
1 nature research | reporting summary October 2018 Corresponding author(s): DBPR 2019-01-00815A Last updated by author(s):May 30 ...
2 nature research | reporting summary October 2018 Field-specific reporting Please select the one below that is the best fit for ...
3 nature research | reporting summary October 2018 #2861354, 1:100), rabbit anti-G3BP1 (13057-2-AP, Proteintech, Lot #00047654, ...
Letter https://doi.org/10.1038/s41586-019-1372-3 Structure and assembly of the mitochondrial membrane remodelling GTPase Mgm1 Ka ...
reSeArCH Letter conformation of dynamin^17 –^19. The Mgm1 stalk domain is shorter than the dynamin stalk, comprising an antipara ...
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