Nature - USA (2020-06-25)

Nature | Vol 582 | 25 June 2020 | 583 between two surfaces, T cell locomotion depends on integrin-mediated force transmission^6 ...

584 | Nature | Vol 582 | 25 June 2020 Article dendritic cells retained a weak locomotive capacity even in smooth channels (Exten ...

Nature | Vol 582 | 25 June 2020 | 585 retrograde actin flow to the surrounding and can therefore operate alternately or even sim ...

Article Methods Cell culture Cells were grown and maintained in a humidified incubator at 37 °C and 5% CO 2. LMR7.5 T cell hybri ...

EDTA device. A 4-μm high master was produced by spin-coating SU8-2005 for 30 s at 5,000 rpm, pre-baking for 2 min at 95 °C and U ...

Article 2.5 μg/ml CCL19 (250-27B, Peprotech) added on the opposite hole of cell entry and with or without 30 mM EDTA^13. Multila ...

Actin flow versus cell velocity analysis. For Figs. 1e–g, 4e–g, Extended Data Figs. 1l, 4b, 5g, 8a, kymographs were generated al ...

Article GAPDH Talin1 and 2 Scram ble Talin1g1Talin1g 2 Talin1g3 a Control Talin KO CRISPR virus infection d0 d1 d3 Media Renewal ...

abc d ControlTalin KO 60:0 (^000204060) 5.10^3 10.10^3 15.10^3 20.10^3 25.10^3 Time (min) MSD (μ m 2 ) Control TalinKO e 0 5 10 ...

Article a Cell speed (μm/min) No chemokine + CCL19 EDTA -+-+- + Channel zone 6-12μm 24μm Smooth Cell speed (μm/min) **** **** ** ...

Actin cortex Cell length TIRF Lifeact-GFP TIRF Actin retrograde flow a 6- m 12- m 24- m Smooth 0 10 20 30 6- m 12- m 24- m Smoot ...

Article a c Cell loading port Outlet Loading port Outlet Confinement channelsMicro- Confinement b f 2.5D Serrated channel Smooth ...

Extended Data Fig. 5 | Actin polymerization versus contractility during topography-based locomotion. a, Scheme of the device use ...

Article Agarose ab 50μm perpendicular to ridges parallel to ridges side view top view 800nm 600 nm 800nm Agarose WT and Lifeact- ...

Extended Data Fig. 6 | Topography-based migration of primary T cells. a, Scheme (top and side view) of control and Lifeact-GFP c ...

Article Actin: optical flow Angle of actin flow (°) Number of detections Angle of cell movement (°) Number of detections e b Det ...

b +-Curvature 0 30 60 0210 0 30 Distance (μm) Front Rear a Time (s) 0 30 60 Left semiperimeter (μm) Right semiperimeter (μm) 30 ...

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