Nature - USA (2020-08-20)

Nature | Vol 584 | 20 August 2020 | 475 Article Structure of the ER membrane complex, a transmembrane-domain insertase Lin Bai^1 ...

476 | Nature | Vol 584 | 20 August 2020 Article yeast cells by fluorescence microscopy (Fig. 1d, Extended Data Fig. 2). The nine ...

Nature | Vol 584 | 20 August 2020 | 477 disulfide bonds were consistent with our membrane orientation assign- ment of the EMC, i ...

478 | Nature | Vol 584 | 20 August 2020 Article to properly fold and locate to the membranes of the Emc3(K26L) cells (Fig. 3f, E ...

Methods No statistical methods were used to predetermine sample size. The experiments were not randomized, and investigators wer ...

Article affinity resin and size-exclusion chromatography using the same protocol as for the EMC. In the binding assay, twice as ...

Extended Data Fig. 1 | Data processing and validation of cryo-EM micrographs and 3D reconstruction. a, Gel filtration profile of ...

Article Extended Data Fig. 2 | Protein abundance and localization of nine putative EMC clients in wild-type and Emc3-knockout ye ...

Extended Data Fig. 3 | Cryo-EM 3D density map of the EMC. a–d, The surface-rendered map is shown in front view (a), left side vi ...

Article Extended Data Fig. 4 | The f itting of the atomic model and the 3D map in selected regions. 3D density map and atomic mo ...

Extended Data Fig. 5 | Structure of the lumenal and cytosolic regions of the yeast EMC. a, Structure of the EMC lumenal region s ...

Article Extended Data Fig. 6 | In vitro binding assays between the purif ied EMC and the TOM complex. a, Gel filtration profiles ...

Extended Data Fig. 7 | The 3D EM map of the EMC surface rendered at a low display threshold. The bound lipids/detergents surroun ...

Article Extended Data Fig. 8 | Structural comparison between yeast EMC and E. coli YidC. a, Structure of EMC in cartoon. b, Stru ...

Extended Data Fig. 9 | Comparisons of protein abundance, localization, and growth of the mutant yeast strains with the wild-type ...

Article Extended Data Table 1 | Cryo-EM data collection, refinement, and validation statisticsCryo-EM data collection, refinemen ...

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Nature | Vol 584 | 20 August 2020 | 479 Article Structure of the essential inner membrane lipopolysaccharide–PbgA complex Thomas ...

480 | Nature | Vol 584 | 20 August 2020 Article (Fig. 1a–c, Extended Data Fig. 1a, Supplementary Table 1). Serum and rifampicin ...