Plant Biotechnology and Genetics: Principles, Techniques and Applications

without selection. With certain traits, such as herbicide resistance, the gene of interest may be directly selectable without th ...

Only a few studies have emerged in which transposons have been used to translocate genes within the plant genome to break the li ...

Saccharomyces cerevisiae, and R/RS from Zygosaccharomyces rouxii [reviewed by Ebinuma et al. (2001)]. The target sequences are p ...

LIFE BOX 9.1. DAVID W. OW David W. Ow, Principal Investigator, Plant Gene Expression Center, USDA–ARS/UC Berkeley David Owwhile ...

come to Boston every so often as his son was doing graduate work at the University of Massachusetts. As his lab also worked on n ...

then has been rewarding and the stories behind them equally interesting. However, the later years were just seg- ments of the jo ...

all things!) convinced me that science might be out of reach after all. My fairly recent (and pregnant) wife and I decided to pa ...

References Bevan MW, Flavell RB, Chilton M-D (1983): A chimaeric antibiotic resistance gene as a selectable marker for plant cel ...

Svab Z, Maliga P (1993): High-frequency plastid transformation in tobacco by selection for a chimeric aadAgene.Proc Natl Acad Sc ...

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&CHAPTER 10 Transgenic Plant Production JOHN FINER and TANIYA DHILLON Department of Horticulture and Crop Science, OARDC/The ...

How do the DNA integration patterns differ in plant cells, transformed via Agrobacteriumand particle bombardment? Can you think ...

and target it to the nucleus? Also, where are the cells that need to be targeted; ones that can either give rise to whole plants ...

10.2.3 Target Tissue Status For successful production of transgenic plants, plant cells, which have the ability to grow (differe ...

Transformed cells can, however, be selected in other ways. “Positive selection” refers to the ability of a cell to survive by ut ...

notably roses, grapes, and euonymus. The main symptom of the disease is a gall or tumor that forms on the cut stem (Fig. 10.1) o ...

clarify parts of the story. If one of the hormone biosynthesis genes was disrupted and the bacterium was inoculated onto tobacco ...

many thorough reviews on the mechanism ofAgrobacteriumT-DNA transfer (Binns and Thomashow 1988; Zambryski 1992; Tzfira and Citov ...

infected region to prevent spread of the invasion. Pathogens, in turn, have developed methods to introduce regulatory compounds ...

The method that may hold the most promise for future increases in efficiency of Agrobacterium-mediated transformation is to alte ...