Plant Biotechnology and Genetics: Principles, Techniques and Applications

will be introgressing the transgenes from this event into a carefully chosen 30 popular southeastern Asian rice var- ieties. Der ...

Fischoff DA et al. (1987): Insect tolerant transgenic tomato plants.Bio/Technol 5 :807–813. Fitch MMM, Manshardt RM, Gonsalves D ...

&CHAPTER 9 Marker Genes and Promoters BRIAN MIKI Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada 9.0. CHAPTER SUMM ...

Why is there a need for negative selectable markers in experimental plant science? Can you design experiments that would employ ...

explants, there would be little chance of recovering transgenic cells without selectable markers. Selectable marker genes fall i ...

plant gene expression machinery. The modifications could include changes to the codons favored by plants and elimination of cryp ...

without the use of external substrates. Both allow the recovery of the transgenic cells that may be present at a very low freque ...

monitor the transmission of the linked transgenes among the progeny of the plant. One hopes for a transgenic line or event with ...

Figure 9.4.TheuidAgene, coding for GUS, as an example of a reporter gene that has been exten- sively used in plants. (a) Histoch ...

pattern conferred by gene regulatory elements fused to the chimeric reporter gene (Fig. 9.1) and how the gene is controlled in t ...

marker gene among progeny. Onlyconstitutivepromoters can ensure expression in all tissues and at all stages of development (Figs ...

have also shown that interactions could occur with infecting viral sequences resulting in transgene silencing. In transgene sile ...

reporter gene linked together in the same DNA fragment that is transferred to the plant cell (Fig. 9.1). As discussed above, the ...

This category includes some recently developed systems that involve genes that provide access to a nutrient source that can be u ...

(El Ouakfaoui and Miki 2005). This gene has been the most extensively studied among the selectable marker genes, and the largest ...

9.4.2 Nonconditional Positive Selection Systems A very new area of research is the development of positive selection systems tha ...

regulatory elements that limit expression within them. As such, it may also be considered as a complement to the reporter genes ...

nontarget or pleiotropic effects (El Ouakfaoui et al. 2005). It is the most frequently used reporter gene in field trials so far ...

GFP requires no external substrate for detection, and there have been no reports of detri- mental effects on the fitness of plan ...

maintain them in the transgenic plant. Their removal may provide some advantages if the plant is to be used for another round of ...