Analytical Chemistry

Table 4.16 Typical applications of thin-layer chromatography Separation Stationary phase Mobile phase amino acids and peptides c ...

Structure of Ion-exchange Resins The most widely used type of resin is a copolymer of styrene and divinyl benzene produced in be ...

The chloromethylation reaction can result in cross-linking additional to that produced by the DVB. Thus for anionic resins, the ...

chemically modified microparticulate silicas (p. 124) incorporating cationic or anionic ion-exchange groups are available. Being ...

exchanging ion and the hydrogen ion within the resin structure. Thus, the greater the affinity for a particular ion, relative to ...

selectivity for monovalent over polyvalent ions increases. Ionic properties which determine resin affinity are complex involving ...

a rate depending on their selectivity coefficients. Sorption isotherms are approximately linear in dilute solutions so that elut ...

the separation of many metals as halide complexes on anionic resins and the separation of amino-acids with citrate buffers. The ...

in aqueous media and in such solvents as ethylene glycol and dimethylformamide. Bio-Gel (a copolymer of acrylamide and N,N'-meth ...

...

and non-compressible are particularly useful in separations where the mobile phase is pressurized as in HPLC (p. 118 et seq). Th ...

Applications of Size Exclusion Chromatography Relative molecular mass distributions for components of biochemical and polymer sy ...

at the centre, separating into individual bands or spots. Detection techniques such as spraying with a chromogenic reagent or st ...

Figure 4.52 Electropherogram of blood serum on cellulose acetate. (Buffer: 0.50 M barbitone, pH = 8.6) Effect of Temperature, pH ...

relative to the position reached by a neutral molecule. Propanone, urea or glucose is sometimes added to a sample for this purpo ...

in detecting an abnormal distribution of components in a series of routine samples. It is used widely for the analysis of biolog ...

Table 4.20 Diffusion coefficients of species of different sizes Species RMM (MW) D (cm^2 s–^1 ) 105 HCl 36 3.05 NaCl 58 1.48 β- ...

Figure 4.54 Schematic diagram of a capillary electrophoresis system. and an o.d. of about 400 μm. Like capillary GC columns, it ...

Table 4.21 Detectors for capillary electrophoresis Method Mass detection limit (moles) Concentration detection limit (molar)a Ad ...

Figure 4.55 Methods of sample injection for capillary electrophoresis. Modes of HPCE Different separation mechanisms, which dete ...