Analytical Chemistry

Table 4.22 Principal modes of HPCE Mode Separation mechanism Separation medium Typical sample types capillary zone electrophores ...

Table 4.23 Buffers commonly used in capillary electrophoresis Name pKa phosphate 2.12 (pKa1) citrate 3.06 (pKa1) formate 3.75 su ...

CAPS 10.40 phosphate 12.32 (pKa3) CZE is finding a wide range of applications, mostly in the biochemical, clinical and pharmaceu ...

Table 4.24 Some additives used in capillary electrophoresis Additive Example Use surfactants (anionic, cationic, or neutral) SDS ...

Figure 4.56 Separation in MEKC. S = solute. or 'eluting' with the same velocity as the micelles. Hydrophilic neutral solutes tha ...

Capillary gel electrophoresis (CGE) is a variation where selectivity based on molecular size and shape is introduced by filling ...

Figure 4.58 Separation by CIEF. points after which they are passed sequentially through the detector by applying pressure at one ...

CEC has a number of advantages over both CE and HPLC. The flat flow profile arising from the EOF gives plate heights of about ha ...

...

Figure 4.59 Some examples of separations by HPCE. (a) CZE BSA peptide map. Conditions: 20 mM phosphate, pH 7, V = 25 kV, i = 16 ...

components. ...

References Craig, L. C. and Craig, D., in Techniques in Organic Chemistry, A. Weissberger (ed.), Vol. III, p. 248, Interscience ...

Problems (1) Compound X has a distribution ratio of 2.7 for benzene/water. For an aqueous sample containing 4.5 mg of X per 50 c ...

n-propane 3.6 propylene 4.3 isobutane 5.5 n-butane 7.5 isobutylene 8.6 trans- 2 - butene 10.6 cis- 2 - butene 12.3 isopentane 13 ...

H (cm) 0.635 0.510 0.423 0.465 0.552 0.632 0.692 0.749 cm s–1) 0.91 1.51 3.0 4.2 5.55 7.0 8.0 9.0 Plot a graph of H versus and c ...

(c) the plate number for each compound using widths at 1/2 -height; (d) the resolution of the two compounds, Rs. (13) Two compou ...

Denny, R. C., A Dictionary of Chromatography (2nd edn), Macmillan, London, 1982. Gilbert, M. T. High Performance Liquid Chromato ...

5 — Titrimetry and Gravimetry Titrimetric and gravimetric analyses, which are largely derived from the application of the princi ...

A titrimetric method involves the controlled reaction of a standard reagent in known amounts with a solution of the analyte, in ...

End-point Detection A prerequisite for a precise and accurate titration is the reproducible identification of an end point which ...