Science - USA (2022-02-25)

RESEARCH ARTICLE ◥ NEUROSCIENCE Hyperexcitable arousal circuits drive sleep instability during aging Shi-Bin Li1,2†, Valentina M ...

indicated by the cutaway surface (Fig. 2, D and J) for the mean value of wake duration in aged mice (Fig. 2, F and L). These res ...

We next compared light-evoked firing activ- itybetweentheyoungandagedHcrtneurons expressing ChR2-eYFP. We tested different sti- ...

sgKcnq2/3 viral mixture for disruption of Kcnq2/3genes (Fig. 5A). All the mice were implanted with EEG-EMG electrodes for sleep/ ...

beginning of light phase after the familiar- ization session in the object-recognition task also improved the subsequent explora ...

well-being in elderly individuals ( 3 ). However, the mechanistic underpinnings of aging-related sleep fragmentation are unknown ...

M-current with a key role in neuron repolari- zation may also contribute to increased excit- ability of arousal-promoting circui ...

durations in aged mice with the same opto- genetic stimulations in young and aged mice (fig. S13). Electrophysiological recordin ...

the LH (AP:−1.35 mm, ML: ± 0.95 mm, DV: −5.15 mm) of young (3 months) and aged (18 months) male and female Hcrt::Cre mice [3 mic ...

with a concentration of 0.2 mg/ml for in vivo experiment with a dosage of 2 mg/kg (0.1 ml/ 10 g, intraperitoneally). 5 mM flupir ...

prepared and imaged in as similar conditions as possible, all samples were paired starting at the tissue preparation step. Thus, ...

CAGFLEXmCherry(sgControl)werepack- aged into AAV-DJ by the Wu Tsai Neuro- sciences Institute Gene Vector and Virus Core at Stanf ...

tendency.Neurobiol. Aging 3 , 321–327 (1982). doi:10.1016/ 0197-4580(82)90020-3; pmid: 7170049 B. A. Mander, J. R. Winer, M. P. ...

V.M.D.’s work. G.S. supervised C.C.’s work. L.d.L. supervised the entire project. S.-B.L. prepared figures and wrote the manuscr ...

RESEARCH ARTICLES ◥ PROTEIN TARGETING Mechanism of signal sequence handover from NAC to SRP on ribosomes during ER-protein targe ...

840 25 FEBRUARY 2022¥VOL 375 ISSUE 6583 science.orgSCIENCE Fig. 1. Structure of the ribosome¥NAC complex reveals interactions be ...

hydrophobic ER-targeting signals somehow weaken the interaction between the NAC glob- ular domain and the ribosome to allow SRP ...

chain, as evidenced by cross-linking between a residue in the NACbanchor and the ribo- somal protein eL22, whereas a probe in th ...

To address this, we generated the following: (i) a NAC mutant in which the UBA is deleted (dUBA), (ii) a NAC mutant (D205R/N208R ...

ER signal sequence weakens the interactions of NAC’s globular domain with the ribosome. This allows SRP to bind the signal seque ...