Biophotonics_Concepts_to_Applications
indicate a better ability to distinguish two closely spaced points. Resolution also depends on the wavelength of the illuminatio ...
Thelimit of resolutionrefers to the smallest separation between two closely spaced Airy disks for which a microscope objective c ...
Example 8.6Consider two microscope setups being used at a 700 nm wavelength. Suppose for one setup NAobj1= 0.80 and for the othe ...
and other in front of the photodetector. With this setup, the focal plane of the specimen and the image plane at the retina of t ...
sample returns through thefiber bundle, passes through the scanning and dichroic mirror system, and travels to the photodetector ...
fluorescence microscopy is a technique for viewing specimens that aredyed(also referred to asstained),labeled,marked,ortaggedwit ...
An excitation optical filter, which passes only the selected spectral band (≈40 nm wide) needed for absorption by thefluorophor ...
and higher photon excitations are also possible if the density of the excitation photons is high enough to ensure a sufficient l ...
A key application of multiphoton microscopy has been for noninvasive imaging deep within scattering media, such as biological ti ...
8.7 Light Sheet Fluorescence Microscopy. As described in the sections above, in a conventional microscope a sample is illuminate ...
sample by using a plane of light instead of a point as in confocal microscopy, LSFM can acquire images at speeds ranging from 10 ...
microscopic techniques can visualize objects ranging in size from millimeters to nanometers. This chapter addressed the basic fu ...
mechanism focuses the light sequentially onto each individualfiber in the coherent bundle, so that the light gets transmitted to ...
U. Kubitscheck,Fluorescence Microscopy: From Principles to Biological Applications (Wiley-Blackman, Weinheim, 2013) Y. Engelbor ...
Chapter 9 Spectroscopic Methodologies Abstract Numerous viable optical spectroscopic methodologies are being imple- mented in bi ...
Rapid diagnosis of bacterial infections in blood samples to determine the correct selection and administration of antibiotics M ...
(Greek letter nu) of electromagnetic radiation is defined byυ=1/λ, with typical units being cm−^1. Thus, for example, the wavenu ...
As Sects.6.7and8.4describe, certain types offluorophores within molecules have the ability to absorb external excitation light a ...
Several relaxation transition possibilities then can take place to return the molecule back to the S 0 state from the S 1 state. ...
resonance energy transfer(RET), orelectronic energy transfer(EET). The FRET technique is used influorescence microscopy and mole ...
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