Stem Cell Processing (Stem Cells in Clinical Applications)

© Springer International Publishing Switzerland 2016 131 P.V. Pham (ed.), Stem Cell Processing, Stem Cells in Clinical Applicati ...

132 Stem cells should meet various criteria before being considered for regenerative medicine purposes. The cells should (1) be ...

133 The isolation of ASCs from adipose tissue was first pioneered in the 1960s. Minced rat fat pads were extensively washed to r ...

134 Cell counts may be obtained by using either a manual method such as the trypan blue (0.4 %) dye exclusion method or automate ...

135 (Both et al. 2007 ). The cells seeded at the lower density reached the target of 2 × 10^8 cells in total in a shorter time p ...

136 Xeno- and Serum-Free Cell Expansion for Clinical Application Synthetic, chemically defined serum-free medium has been recomm ...

137 7.2.2 Isolation Using Automated Closed Systems An automated closed system allows for minimal handling of a sample by the ope ...

138 contaminants. The Celution® system automatically calculates the amount of Celase® enzyme required for proper digestion of th ...

Table 7.2 A summary of marker expression profiles and known physiological functions of the more common surface antigen markers u ...

14 0 cells should co-express the following markers: CD13, CD29, CD36, CD44, CD73, CD90 and CD105 (Bourin et al. 2013 ). There sh ...

141 forming mature adipocytes (Tang et al. 2004 ). Peroxisome proliferator-activated receptor-γ (PPAR-γ) and CCAAT/enhancer-bind ...

142 phosphodiesterase activity causing an increase in intracellular cAMP levels by stimulat- ing the cAMP-dependent protein kina ...

143 Assessment of Adipogenic Differentiation Adipogenic differentiation may be measured using both quantitative and qualitative ...

14 4 There are also several other hydrophobic fluorescent dyes available that may be useful in the quantitative assessment of ad ...

145 7.3.2.2 Osteogenesis There appears to be a complex relationship between adipogenesis and osteogen- esis. Both adipocytes and ...

Fig. 7.4 Flow cytometric analysis of adipocyte differentiation of ASCs after staining with Nile Red (20 ng/ml final concentratio ...

147 7.3.2.3 Chondrogenesis Cartilage is a specialized connective tissue which consists of chondrocytes surrounded by an ECM. Cho ...

148 BMP-2, -4, and -6 with regard to their ability to enhance in vitro formation of cartilage demonstrated that BMP-2 was the mo ...

149 7.3.3 Experimental Animal Models In order to reach the long-term aim of clinical translation of cell-based therapy, preclini ...

150 Appendix: Brief Description of Current Methods Manual Isolation Procedure The isolation protocol is adapted from the procedu ...