The Lotus japonicus Genome
20 Forward and Reverse Genetics: The LORE1 Retrotransposon Insertion Mutants Eigo Fukai, Anna Małolepszy, Niels Sandal, Makoto H ...
20.2 Mutagens and Identification of the Mutations Forward genetic screening for mutant phenotypes followed by gene cloning using ...
20.3 Legume Mutant Collections From the very beginning of the history of genetics, marked by Mendel’s work on garden peas, legum ...
element, a single clonally amplified founder plant is sufficient to generate a mutant population of any desired size. Another ad ...
lines are ordered through Legume Base (http:// http://www.legumebase.brc.miyazaki-u.ac.jp/lore1Browse Action.do), whereas the Da ...
Fukai E, Dobrowolska AD, Madsen LH, Madsen EB, Umehara Y, Kouchi H, Hirochika H, Stougaard J (2008) Transposition of a 600 thous ...
Xu M, Brar HK, Grosic S, Palmer RG, Bhattacharyya MK (2010) Excision of an active CACTA-like transpos- able element fromDFR2caus ...
21 TILLING inLotus japonicus Trevor L. Wang and Fran Robson Abstract Following the seminal work on TILLING inArabidopsis thalian ...
an allelic series of mutants that permits a refined analysis of gene function, in contrast to knockout mutants. The mutagens mos ...
population to screen for their own particular phe- notypes, such as root architecture and, in particu- lar, spontaneous nodulati ...
21.4 The Process TILLING can be carried out using a range of different technologies, but the central principle is the same: the ...
sequencer (Le Signor et al. 2009 ). Potential mutations are visualised as anomalous‘empty’ peaks on overlaid chromatograms using ...
Table 21.3 Mutations identi fied in Lotus japonicus genes targeted by TILLING Description Genesymbol Predicted genelocation Rele ...
Table 21.3 (continued) Description Genesymbol Predicted genelocation Release 3.0predicted gene Eco-type pre- screen Mis-sense Pr ...
Table 21.3 (continued) Description Genesymbol Predicted genelocation Release 3.0predicted gene Eco-type pre- screen Mis-sense Pr ...
Table 21.3 (continued) Description Genesymbol Predicted genelocation Release 3.0predicted gene Eco-type pre- screen Mis-sense Pr ...
Table 21.3 (continued) Description Genesymbol Predicted genelocation Release 3.0predicted gene Eco-type pre- screen Mis-sense Pr ...
Table 21.3 (continued) Description Genesymbol Predicted genelocation Release 3.0predicted gene Eco-type pre- screen Mis-sense Pr ...
Table 21.3 (continued) Description Genesymbol Predicted genelocation Release 3.0predicted gene Eco-type pre- screen Mis-sense Pr ...
21.6 The Future For more than 10 years, TILLING in Lotus has been the predominant reverse genetic platform for this species, pri ...
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