Quorum Sensing
low concentrations (pM–μM ranges) techniques that can accurately capture their binding profile in a similar range would be great ...
binding of GBLs to their cognate receptor family of proteins remains elusive as they are difficult to purify and there is a gene ...
GBLs. The beauty of this method lies in exploring the structure in its innate form, without having to attach extrinsic fluoropho ...
In order to ascertain the entry of the ligand in the pocket of the regulatory domain and the ligand binding capacity of CprB, we ...
2 Materials (SeeNotes 1 and 2) Milli-Q water is used throughout. Buffers are stored at 4C and protein stocks at 80 C. Luria- ...
3 Methods 3.1 Protein Expression and Purification The clone of native CprB in the pET26b(+) expression vector was a gift to us ...
3.2.1 Instrument Setup 1. Turn on the spectrofluorimeter. Depending on the instrument, several minutes could be required in orde ...
from the binding pocket and will enable to delineate the con- tribution of fluorescence quenching by W185 from that of the trypt ...
deduced. In static quenching, formation of a nonfluorescent ground-state complex occurs between the fluorophore and the quencher ...
3.4.3 Data Analysis 1. The decay obtained is fitted by the global reconvolution fitting technique using the software FluoFit (Gl ...
As fluorescence measurements are sensitive to temperature it is ideal to use thermostatted cell holders. Ideally measure absorb ...
membrane-interactions of amyloidogenic pro- teins. Biochim Biophys Acta 1768:1862–1885 Yengo CM, Chrin L, Rovner AS, Berger CL ...
Chapter 12 Methods to Study Solo/Orphan Quorum-Sensing Receptors Vittorio Venturi, Sujatha Subramoni, Anice Sabag-Daigle, and Br ...
The purpose of this chapter is to describe simple ways to identify these LuxR solos, detect their ligands, and study their regul ...
terminus and a helix-turn-helix DNA-binding domain at their C-terminus. LuxR solos have so far been implicated in intraspecies, ...
we describe a simple method to experimentally confirm ligand binding specificities of the LuxR solo protein. Finally, a method t ...
SDS-PAGE sample buffer: 0.09 M Tris–HCl pH 6.8, 20% (vol/vol) glycerol, 2% (wt/vol) sodium dodecyl sulfate (SDS), 0.02% (wt/vol ...
autoinducer-binding domain” at theN-terminal end. Examine protein sequences positive for IPR005143 for the presence of any of th ...
LuxR solo types. Here we describe steps to distinguish and meth- ods to identify AHL-binding LuxR solos,plant-associatedbacteria ...
of the His-tagged LuxR solo inE. coliin the presence and absence of AHLs or plant extracts and for testing their solubility and ...
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