Quorum Sensing
The concentration range depends on the limit of detection (LOD). It might be necessary to adjust it to the analytical instrumen ...
Ultracentrifugation is only needed if HSLs/HSs should be quantified in intact cells. A detailed description is given by [12]. F ...
Li X, Fekete A, Englmann M, Go ̈tz C, Rothbal- ler M, Frommberger M et al (2006) Develop- mentandapplicationofamethodfor theana ...
Chapter 6 Biosensors for the Detection and Quantification of AI-2 Class Quorum-Sensing Compounds Sathish Rajamani and Richard Sa ...
2 (BAI-2, (2S,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran- borate). These QS compounds regulate the expression of genes inv ...
Strain BL21 (luxS–)carrying plasmid constructs pQE30-CLPY (wild-type LuxP biosensor), LuxP mutant biosensors: pQE30- M2CLPY (Q7 ...
Solution C: 50% Glycerol in water, autoclave. Solution D: 0.1 M Arginine dissolved in water, filter sterilize. To 960 ml of Solu ...
Spectrofluorometer with cuvette holder and integrated with analysis software. Ultrasonic cell homogenizor/sonicator fitted with ...
2.5 Boron-Depleted Media and Conditioned Media Preparation Amberlite®IRA743 borate-specific chelating resin. Chromatography col ...
analysis. The remaining culture is induced for CLPY expression by adding 0.3 mM IPTG and grown for additional 6 h. After 6.0-h ...
Add 1.0 ml of diluted dye reagent to clean-dry test tubes; add 20 μl of each standard and CLPY fraction solution and vortex for ...
3.3 Biosensor Characterization and Fluorophore Maturation Fluorescence measurements are carried out at room temperature using sp ...
A ligand-binding independent increase in FRET is observed with recently purified biosensor (Fig.2b). This is due to the delayed ...
eliminate any visible cell clumps. The dilutions are made in fresh LM medium and different volumes are plated out on LM agar (se ...
Fig. 3Quantification of BAI-2 fromVibrio harveyicultures using the CLPY biosensor. (a) Wild-typeV. harveyiBAI-2 levels monitored ...
(Fig.3b,x-axis shown in log 10 scale). The dilution volume for each data point is determined by dividing the volume of condition ...
bright nickel blue), the resin can be cleaned and recharged with nickel for reuse. Following is a modified procedure from Qia- g ...
To have good sample runs clean the wells carefully. Using a pipette set at 100μl and fitted with 1–200μl gel-loading tip, pipet ...
References Lilley BN, Bassler BL (2000) Regulation of quorum sensing inVibrio harveyiby LuxO and sigma-54. Mol Microbiol 36:940 ...
Chapter 7 Detection ofAgr-TypeAutoinducing Peptides Produced byStaphylococcusaureus Ewan J. Murray and Paul Williams Abstract St ...
«
1
2
3
4
5
6
7
8
9
10
»
Free download pdf