Quorum Sensing
agrvaries between differentS.aureusisolates but tends to initialize in mid-exponential phase after the external concentration of ...
great antivirulence potential in vitro, their efficacy in vivo has yet to be determined. Since QS inS.aureusis an excellent mode ...
2.2 Reporter Strain and Plasmids (See Note 1) Strain ROJ48: RN4220Δagr::ErmBpΔagr-lux(integrated as a single crossover); tetrac ...
Inoculate the washed cells (25μl) into 10 ml BHI in a sterile 50 ml Falcon tube. Grow cells at 37C, 200 rpm, and measure optic ...
Use a suitable statistical software package (seeNote 9) to draw a standard curve of AIP concentration relative to maximum RLU/O ...
as an activator of the reporter assay. DNA sequencing of the agrDgene can be utilized to identify the specificagrgroup a strain ...
sensing blocks abscess formation. Proc Natl Acad Sci U S A 102:1691–1696 McDowell P, Affas Z, Reynolds C, Holden MT, Wood SJ, S ...
Chapter 8 Ultra-Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Fa ...
DSF biosensor and the Xcc DSF overproduction mutant ΔrpfC, methods for DSF extraction and purification were estab- lished [5, 6] ...
2.1 Bacterial Cell Cultures Bacterial strain: The methods presented here use therpfC mutant (ΔrpfC) of the Gram-negative bacter ...
Zorbax Eclipse XDB-C18 reverse phase column (Analytical, 4.6150 mm, 5-Micron). 6. 20 C Refrigerator. UPLC/MS system: UPLC sy ...
Evaporate ethyl acetate of the extracts with a rotary evaporator or centrifugal evaporator (maximum temperature 40C) (see Note ...
The concentration of DSF and BDSF in the extract is calculated based on the peak area and the standard curve as shown in Fig.5. ...
This protocol is optimized for measuring the levels of DSF family signals in the cultures ofXccorXanthomonas oryzae pv.oryzae(X ...
The signal intensity or peak area of each of the DSF family signals may vary in different LC-MS systems. We recommend to genera ...
References He YW, Zhang LH (2008) Quorum sensing and virulence regulation inXanthomonas cam- pestris. FEMS Microbiol Rev 32:842 ...
Chapter 9 Rapid Electrochemical Detection ofPseudomonas aeruginosaSignaling Molecules by Boron-Doped Diamond Electrode Alyah Buz ...
for the entire population [8]. PA also utilizes QS for cell-to-cell communication to enable biofilm formation, and regulate the ...
2.1 Electrochemical Workstation The electrochemical cell consists of the BDD electrode (a diameter of 3 mm) as the working elec ...
Electroanalysis is performed by differential pulse voltammetry (DPV) (seeNote 3). Glass beakers (10 or 5 ml). Cleaning the BDD ...
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