Drug Metabolism in Drug Design and Development Basic Concepts and Practice

metabolites are retained somewhat and not eluted in the HPLC solvent front. Since feces displayed the most significant matrix ef ...

is especially useful in HPLC method development and rapid profiling of metabolites with medium-to-low levels of radioactivity. A ...

10.3.3 Dynamic Flow HPLC-RFD A dynamic flow radiodetection technology has been recently developed to enhance the performance of ...

seems to have no problem in maintaining the consistency of the analyte retention times since the total HPLC run time and the HPL ...

injection of 0.25–5 DPM of radioactive materials (Brown et al., 2005, 2006). For example, metabolite profiles of^14 C-R115777, a ...

retention times and peak shapes in a radiochromatogram and its corresponding ion chromatograms recorded from LC–RFD–MS analysis ...

sample. MSC was able to detect and quantify both minor and major metabolites (Fig. 10.7a), which is consistent with the metaboli ...

be significantly reduced or eliminated by the dual stages of chromatographic separation. (3) Higher sensitivity of mass spectrom ...

acquisition of accurate mass spectral data. The online RFD and linear ion-trap MS analyses detected multiple oxidative and conju ...

An example of using off-line HPLC–MSC for profiling plasma metabolites is displayed in Fig. 10.9. Plasma samples containing a to ...

approximately 500 DPM of radioactivity or more are required for detection of minor metabolites (10 DPM, 10 min counting time) co ...

radioactive peaks corresponding to the major buspirone metabolites 3^0 -OH, 5-OH and N-oxide were recovered from the 96-well mic ...

formation rates of the secondary metabolites in the incubations of the individual primary metabolites as determined by radiochro ...

An example of using stop-flow HPLC–RFD to determine the enzyme kinetic parameters of the glucuronidation of muraglitazar in huma ...

TABLE 10.3 Precision and accuracy of stop-flow HPLC/RFD for determining relative radioactivity of muraglitazar and its metabolit ...

rates with substrate concentrations was obtained by the stop-flow HPLC–RFD method (Fig. 10.12a), which was very similar to that ...

chromatography in drug metabolism: validation and applications. J Chromatogr A 2006;1133:184–194. Bu HZ, Kang P, Zhao P, Pool WF ...

Hazai I, Patfalusi M, Klebovich I, Urmos I. Whole-body autoradiography and quantitative organ-level distribution study of deramc ...

Mullen W, Graf BA, Caldwell ST, Hartley RC, Duthie GG, Edwards CA, Lean ME, Crozier A. Determination of flavonol metabolites in ...

Zhang D, Wang L, Chandrasena G, Ma L, Zhu M, Zhang H, Davis CD, Humphreys WG. Involvement of multiple cytochrome P450 and UDP-gl ...