Drug Metabolism in Drug Design and Development Basic Concepts and Practice

TABLE 12.11 Metabolite identification by using NMR spectroscopy.Metabolicreaction Metabolite identification examples NMR indicat ...

Aromatic hydroxylation(Gerhard et al.,2003) NNH O O HN F NH N O O HN F OH 2 4 The hydroxylation position at C2 or at C4 was no ...

TABLE 12.11 ( Continued ) Metabolicreaction Metabolite identification examples NMR indications Confirmation method and comments ...

Glutathione conjugation(Dagnino-Subiabreet al., 2000) HO HO NH 2 NH 2 NH 2 HO HO S O HN HO O NH O OH O The C-5 aromatic proton ...

TABLE 12.11 ( Continued ) Metabolic reaction Metabolite identification examples NMR indications Confirmation method and comments ...

13.Amino acidconjugation OH O NH O OH O Glycine Hippuric Acid Benzoic Acid The proton signal for the methylene proton wasat d4.3 ...

spectral data sets led to the establishment of the metabolite structure as 6-hydroxy buspirone. 12.8 EXAMPLES OF METABOLITE STRU ...

two methylene proton signals for the cyclopropyl ring (two triplets in 0.2– 0.4 ppm range) were missing. Third, the TOCSY experi ...

Basker MJ, Finch SC, Tyler JW. Use of^1 HNMR in the identification of a metabolite of a catecholic cephalosporin excreted in rat ...

Friebolin H, Basic One- and Two-Dimensional NMR Spectroscopy. fourth ed. John Wiley & Sons; 2005. Froehlich AK, Girreser D, ...

Munro SLA, Craik DJ. NMR conformational studies of fenamate non-steroidal antiinflammatory drugs. Magn Reson Chem 1994;32(6):335 ...

Sridharan V, Saravanan S, Muthusubramanian S, Sivasubramanian S. NMR investiga- tion of hydrogen bonding and 1,3-tautomerism in ...

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PART IV COMMON EXPERIMENTAL APPROACHES AND PROTOCOLS ...

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13 DETERMINATION OF METABOLIC RATES AND ENZYME KINETICS ZHI-YIZHANG ANDLAURENCES. KAMINSKY 13.1 INTRODUCTION In vitrobiochemical ...

prediction of human metabolic clearance, such asKmandVmax,needtobe accurately determined. The features of metabolic stability st ...

degradations can take place in both blood and liver, since the latter also expresses several classes of hydrolyases, such as car ...

the standard enzyme source used in metabolic stability studies. However, thein vitrosystem that uses liver S9 fractions, which c ...

Factors of Concern Pharmacological or physiological relevance is always a concern forin vitroexperiments, including microsomal s ...