Front Matter

Han, D., Rhee, J.S., Lee, S.B. (1987b), Lipase reactions in AOT-isooctane reversed micelles: effect of water on equilibria.Biote ...

Kuboi, R., Yamada, Y., Komasawa, I. (1992). Effect of water content on the activity of lipase-hydrolysis of olive oil in reverse ...

Oh, S.G., Holmberg, K., Ninham, B.W. (1996), Effect of hydrocarbon chain length on yield of lipase catalyzed triglyceride synthe ...

Rodakiewicz-Nowak, J., Maslakiewicz, P., Haber, J. (1996), The effect of linoleic acid on pH inside sodium bis(2-ethylhexyl)sulf ...

Tsai, S.W., Chiang, C.L. (1991), Kinetics, mechanism, and time course analysis of lipase-catalyzed hy- drolysis of high concentr ...

4 Cloning, Mutagenesis, and Biochemical Properties of a Lipase from the Fungus Rhizopus delemar Michael J. Haas, David G. Bailey ...

position and arrangement mimic those of human milk fat lipids (Quinlan and Moore, 1993). However, at the time that the research ...

ing resin had a high affinity and capacity for lipase. A small column (20 mL bed volume) quantitatively bound lipase from 20–30 ...

Among the most useful information obtained from the purified lipase was amino acid sequence data. The sample was sufficiently pu ...

The resulting three-dimensional model of the lipase (Figure 3) showed an extre- mely high degree of homology with the similarly ...

substrate (Brzozowski et al., 1991; Derewenda et al., 1992). This was proposed to be the functional basis of ‘interfacial activa ...

solution, can affect the lid conformation. The lid appears to be in dynamic motion, exposing the active site even in the absence ...

plaques were noted. Analysis of one of these indicated the presence of an approxi- mately 1.3 kilobase DNA insert that was subcl ...

DNA sequence identity within the signal, propeptide, and mature lipase regions were 47 %, 53 % and 59 %, respectively. Maximum a ...

in subcloning the lipase genes into expression plasmids, especially those that had high copy numbers, extra precautions were tak ...

4.7 Probing the role of structure-function relationships in substrate selectivity Identification of the relationship between pri ...

simulations were conducted to identify alterations in amino acid sequence that might impact substrate specificity. Appropriate m ...

that of its single-mutant progenitors (Klein et al., 1997a). However, not surprisingly, the introduction of these large amino ac ...

solid base of knowledge that makes lipases one of today’s best characterized and most frequently employed biocatalysts. Addition ...

Kouker, G., Jaeger, K.-E. (1987), Specific and sensitive plate assay for bacterial lipases,Appl. Environm. Microbiol. 53 , 211–2 ...