Front Matter
5 Molecular Basis of Specificity and Stereoselectivity of Microbial Lipases toward Triacylglycerols Ju ̈rgen Pleiss 5.1 Biochemi ...
chum candidumlipase II, was reversal of stereopreference observed when triolein was used as substrate instead of trioctanoin. In ...
4. Other yeast lipases:Candida rugosa(1CRL,1LPM, 1LPN, 1LPO, 1LPP, 1LPS, 1TRH),Geotrichum candidum(1THG), and the structurally s ...
pocket at the bottom of the active site crevice, and the two dibutyl-carbamoyl chains pointing towards the surface of the protei ...
5.3 Modeling and engineering of fatty acid specificity 89 Figure 1. Comparison of the shape of binding pockets ofRhizomucor mieh ...
binding pocket and is positioned near C 4 of the scissile fatty acid chain. Replacing it by the hydrophilic threonine decreases ...
Candida antarcticalipase B (CALB) is a member of the funnel-like lipases (Pleiss et al., 1998). Its binding pocket is an ellipti ...
5.4 Modeling and engineering of stereoselectivity To date, there is no general rule to explain thesn-1(3) stereopreference of al ...
acid chain binds to the hydrophobic crevice and is, subsequently, cleaved off; and in sn-3 orientation, where thesn-3 fatty acid ...
94 5 Molecular Basis of Specificity and Stereoselectivity of Microbial Lipases Table 2.Stereopreferences of lipases fromRhizopus ...
The side chain of L258 was identified as the major factor to discriminate between sn-1 andsn-3 orientation. To verify and apply ...
in stereoselectivity result from residues which do not directly contact the substrate, but have an indirect impact on the bindin ...
Berger, M., Schneider, M.P. (1991), Lipases in organic solvents: The fatty acid chain length profile, Biotechnol. Lett. 13 , 641 ...
Lawson, D.M., Brzozowski, A.M., Rety, S., Verma, C., Dodson, G.G. (1994), Probing the nature of sub- strate binding inHumicola l ...
Uppenberg, J., Ohrner, N., Norin, M., Hult, K., Kleywegt, G.J., Patkar, S., Waagen, V., Anthonsen, T., Jones, T.A. (1995), Cryst ...
6 Lipase-Catalyzed Synthesis of Regioisomer- ically Pure Mono- and Diglycerides B. Aha, M. Berger, B. Jakob, G. Machmu ̈ller, C. ...
6.2 Partial glycerides Mixtures of long-chain monoglycerides and other partial glycerides of varying com- position and constitut ...
ides could provide numerous advantages as compared to the above-described, con- ventional methods such as increased selectivity, ...
nent or temporary protection groups. In this sense, two general routes to the desired molecules are available in principle (Figu ...
which contain up to 50 % (w/w) of glycerol. The new compositions of matter ob- tained in this way behave completely different fr ...
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