AMPK Methods and Protocols

dis-attachment from the plates with a low trypsin concentra- tion (0.05%) to minimize damage to exposed substrate trans- porter ...

(3):928–935. https://doi.org/10.1172/ JCI113700 Luiken JJ, van Nieuwenhoven FA, America G, van der Vusse GJ, Glatz JF (1997) Up ...

(4):709–717. https://doi.org/10.1194/jlr. M023424 Luiken JJ, Glatz JF, Neumann D (2015) Car- diac contraction-induced GLUT4 tra ...

Rajamohan F, Reyes AR, Frisbie RK, Hoth LR, Sahasrabudhe P, Magyar R, Landro JA, Withka JM, Caspers NL, Calabrese MF, Ward J, K ...

Chapter 23 Measurement of AMPK-Induced Inhibition of Lipid Synthesis Flux in Cultured Cells Marc Foretz and Benoit Viollet Abstr ...

pathological conditions characterized by abnormal hepatic lipid accumulation, such as nonalcoholic fatty liver disease (NAFLD) [ ...

Water-saturated petroleum ether (boiling point at 40–60C) (seeNote 10). Bromophenol blue stock solution: 0.2% (w/v) bromopheno ...

Cap the tubes and vortex for 1 min. Centrifuge at 1000gfor 2 min to separate aqueous and organic phases (seeNote 16). Transfer ...

control to normalize data. If the cells studied are available in small numbers, the protocol can be adapted to 12- or 24-well pl ...

Fig. 1Effects of metformin and small-molecule 991 on lipid synthesis rates in WT and AMPK-deficient hepatocytes. After plating, ...

[1-^14 C]-labeled acetate concentration in medium is 0.6μCi/ mL, and cold acetate concentration is 0.6 mM. Non-saponifiable fra ...

Acknowledgments Work from the authors was performed within the De ́partement Hospitalo-Universitaire (DHU) AUToimmune and HORmon ...

Ford RJ, Fullerton MD, Pinkosky SL, Day EA, Scott JW, Oakhill JS, Bujak AL, Smith BK, Crane JD, Blumer RM, Marcinko K, Kemp BE, ...

Chapter 24 Studying the Role of AMPK in Autophagy Sarah Krieg, Bernhard L€uscher, Jo ̈rg Vervoorts, and Marc Dohmen Abstract AMP ...

Autophagy relies on a tightly regulated and interconnected cascade of protein complexes encoded by more than 30 ATG (autophagy-r ...

of AMPK for the induction of autophagy in response to a stimulus of interest. In the following we describe the protocols that ha ...

Transfection reagent. Store at 4C. 5 siRNA buffer (Dharmacon, Cat #B-002000-UB-100). Store at 4C. Dilute sterilely 1:5 with ...

Semidry blotting apparatus. Tank blotting system. Store cooling element at 20 C. Tris-buffered saline (TBS): 50 mM Tris–HCl, ...

Goat anti-guinea pig IgG (HþL) HRP-conjugated (Santa Cruz, sc2438): for IB 1:5000 in TBS-T, 5% (w/v) skim milk powder. Rat anti ...

Seed 1.2 106 cells, counted using a cell counter, onto a 10 cm dish containing a glass coverslip to be able to perform immu- n ...