AMPK Methods and Protocols

Cell Treatment Proceed to AMPK modulation, by using pharmacological approaches (seeTable4 for further details). Alternatively i ...

Distribute each intermediate solution for glycolysis assay prepared as in Subheading2.3,items 6– 8 , in the sensor car- tridge ...

3.6 Equilibration 1. Open the assay template previously configured (seeSubheading 3.3) on the Seahorse Wave software. Start the ...

Save the results. The results can be exported in Prism format for further analysis. Alternatively the results can be exported i ...

Fig. 5Mitochondrial respiration analysis in the presence of various energy substrates. Mitochondrial respira- tion profile of pr ...

capacity. Due to their inhibiting action on the complex of the electron transport chain, the addition of Rot/AA totally inhibits ...

We determined the optimal cell confluence via an optimization test (Fig.3). As the cell density can vary according to the cell ...

dependent on glucose metabolism. We invite the reader to perform a similar test to verify the glycolytic profile of the cells to ...

AMP-activated protein kinase (AMPK) is asso- ciated with enhancement of Glut1-mediated glucose transport. Arch Biochem Biophys 3 ...

Xiao B, Sanders MJ, Carmena D, Bright NJ, Haire LF, Underwood E, Patel BR, Heath RB, Walker PA, Hallen S, Giordanetto F, Martin ...

Chapter 20 Investigating the Role of AMPK in Inflammation Sarah J. Mancini and Ian P. Salt Abstract In addition to the well-char ...

microscopy is an effective way to assess the localization of these proteins following exposure to test substances. Cells are fix ...

LDL-cholesterol, leading to the formation of “foam cells” and development of atherosclerotic plaque [20]. The degree of mono- cy ...

which must be used as a vehicle control in experiments. Activation of AMPK by A769662, Compound 991, and AICAR is cell type- spe ...

glucose. A 10 solution without CaCl 2 or glucose can be prepared and stored at 4C. CaCl 2 and glucose can be added to a 1solu ...

or 3T3-L1 adipocytes and localizing phosphorylated JNK are found in the Subheading4. Grow cells to confluence on 13 mm diameter ...

Spot 35μl of rabbit anti-NFκB (p65) antibodies diluted 1:50 in IF buffer for each coverslip onto parafilm (seeNotes 14and 15 ). ...

3.2 Preparation of Conditioned Medium from Endothelial Cells for the Analysis of Cytokine and Chemokine Secretion Grow human en ...

3.3 Monocyte Adhesion Assay Grow endothelial cells to confluence on 24-well plates—4- wells per condition to give four technica ...

Paraformaldehyde is a carcinogen, therefore gloves and a mask should be worn when preparing this solution. Removing serum in th ...