AMPK Methods and Protocols

3.4 Semi-dry Transfer Prepare the filter paper (extra-thick blot paper) and submerge it into transfer buffer 1. Activate the P ...

4 Notes After adjusting the pH, the solution turns to pale green color. If stored at 4C, use the reagent within 15 days. You c ...

lasting approximately 7 days. It is mandatory to daily check the food intake and body weight. The specificity of dissection can ...

Two ways to represent protein data are used in literature, namely, correction by non-phosphorylated protein levels and by a pro ...

Steinberg GR, Michell BJ, van Denderen BJ et al (2006) Tumor necrosis factor alpha- induced skeletal muscle insulin resistance ...

protein kinase. Diabetes Obes Metab 15:1128–1135 Skrede S, Martins L, Berge RK, Steen VM, Lopez M, Ferno J (2014) Olanzapine de ...

Chapter 28 Using Ex Vivo Kidney Slices to Study AMPK Effects on Kidney Proteins Renee Rao, Kazuhiro Omi, Roshan Rajani, Hui Li, ...

trying to uncover links between metabolism and transport, espe- cially in kidney epithelial cells. One of the important results ...

quickly activated if samples are not properly handled, many researchers harvest the kidney following specific protocols to pre- ...

Ringer solution: 120 mM NaCl, 25 mM NaHCO 3 , 3.3 mM KH 2 PO 4 , 0.8 mM K 2 HPO 4 , 1.2 mM MgCl 2 , 1.2 mM CaCl 2 , 10 mMD-gluc ...

For mice preparations, use lighter pressure or the kidney tissue, and/or a thinner cover (based on the original cover depicted ...

Cryostat (to slice to 4μm; 30 C). 53.þ/þpoly-L-lysine coated glass slides. Diamond pen for labeling of the glass slides with ...

Using scissors, make an incision in this ventral skin from the direction caput to cauda along the central abdominal line (xipho ...

Place two additional incisions in the abdominal muscle layer perpendicular to the initial xiphoid to pelvis incision toward the ...

Place one of these thick sections on the Stadie-Riggs slicer (Fig. 3a–h) and quickly obtain a thin kidney tissue slice (~0.5 mm ...

Keep the kidney slices in the containers either overnight at 4C or at room temperature for 4 h. To proceed with the kidney sli ...

Placing the kidney slices once for 15 min in quenching buffer (quench step). Rinse tissue again three times, each time for 15 m ...

Prepare tissue lysis buffer (5 mL is sufficient for two whole mouse kidneys). Weigh empty sample tube, and then determine tissu ...

would be an important confounder in evaluating the effects of AMPK activation on cellular function, as it would act as an AMPK a ...

Do not microwave “sugar” containing solutions as the micro- wave process may “caramelize” the solution. This procedure is optio ...