Basic Concepts in Clinical Biochemistry-A Practical Guide.7z

28.4 Reagents........................................ 1.Starch substrate solution: Dissolve 75 mg of starch in about 20 ml of di ...

28.7 Clinical Significance............................... Normal amylase activity ranges from 80 to 180 SU/dl in serum and from ...

To Estimate the Activity of Lipase in Serum 29 29.1 Theory Lipase is the enzymes that cause hydrolysis of glycerol esters of lon ...

29.4 Reagents........................................ 1.Olive oil emulsion (50%): Take equal parts of olive oil and 5% aqueous s ...

Qualitative Analysis of Ketone Bodies in Urine^30 30.1 Theory The three compounds, namely, acetone, acetoacetic acid, andβ-hydro ...

30.2 Rothera’s Test for Acetoacetic Acid and Acetone 30.2.1 Principle Alkaline nitroprusside reacts with keto-group of acetone a ...

30.2.4 Result Appearance of a purple/red ring at the junction of the two layers within 30–60 s indicates the presence of acetone ...

30.5 Detection ofβ-Hydroxybutyrate 30.5.1 Principle β-hydroxybutyrate forms acetoacetate on oxidation with H 2 O 2 which can be ...

Qualitative Test for Bile Pigments and Urobilinogen in Urine^31 31.1 Theory Bile pigments are breakdown products of the blood pi ...

31.2.1.2 Reagents (a) 33% v/v aqueous solution of acetic acid. (b) 10% aqueous solution of BaCl 2. (c) Fouchet’s reagent: Dissol ...

31.2.2 Hunter’s Test 31.2.2.1 Principle Bilirubin present in the urine sample gets adsorbed on barium sulfate precipitates. This ...

31.3 Urobilinogen in Urine 31.3.1 Ehrlich’s Test 31.3.1.1 Principle A red-colored complex is formed when urobilinogen reacts wit ...

31.5 Clinical Significance............................... Normally small amount of urobilinogen is excreted through urine (1–3.5 ...

Determination of Total Lactate Dehydrogenase Activity in Serum Sample^32 32.1 Theory Lactate dehydrogenase (LDH) is an enzyme in ...

LactateþNADþ ⇄ LDH pyruvateþNADHþHþ 32.4 Reagents........................................ Buffer substrate solution (consisting ...

To Measure Activity of Creatine Kinase in Serum^33 33.1 Theory Creatine kinase (CK), also called creatine phosphokinase, exists ...

N-acetylcysteine, thioglycerol, dithiothreitol, cysteine, etc. The reaction sample is also added with an antibody specific to CK ...

¼ total volume in cuvette ðÞmolar extinction coefficient of NADPH volume of serum used mlðÞ ¼ 1 : 05 6 : 22  10 ^3  0 : 05 ¼ ...

Analysis of Cerebrospinal Fluid for Proteins and Sugars^34 34.1 Theory Cerebrospinalfluid (CSF) is a clear and colorlessfluid th ...

about 850 ml of distilled water. Then add 25 mg of pyrogallol red dye, and mix well to dissolve it completely. Make thefinal vol ...