Basic Concepts in Clinical Biochemistry-A Practical Guide.7z

To Perform Qualitative Test for Reducing Substances in Urine^11 11.1 Theory Sugars may be called as reducing or nonreducing base ...

CuSO 4 Blue þ2H 2 O! sugar Cu OHðÞ 2 white þH 2 SO 4 2Cu OHðÞ 2 heat! Cu 2 O Cuprous oxide ðÞredor yellow þ2H 2 OþO 11.2.2 Bened ...

reacts with the chromogen to form a colored product. Sensitivity of the test is 0.01–1 g/dl. GlucoseGOD!Gluconic acidþH 2 O 2 H ...

Fructose: It may be found in urine after taking food rich in fructose (fruits, honey, syrup, and jams). It may be found in live ...

Quantitative Analysis of Reducing Sugars in Urine^12 12.1 Theory Examination of urine for glucose is rapid and inexpensive and c ...

the precipitation of cuprous oxide and keeps the cuprous ions in solution. Na 2 CO 3 prevents destruction of sugar by providing ...

If the urine sample used is less than 2 ml for the titration, dilute urine 1 in 5 or 1 in 10 with distilled water and include t ...

Estimation of Blood Glucose Levels by Glucose Oxidase Method^13 13.1 Theory The sugar glucose is an aldohexose and is the most i ...

13.3 Specimen Requirements Plasma is the preferred sample for glucose estimation. Separate plasma from cells rapidly to avoid gl ...

13.6 Calculations Plot a graph between amount of glucose at x-axis and absorbance at y-axis. Extrap- olate glucose amount from t ...

Fig. 13.1 (a) Glucose reabsorption in normal conditions and (b) glucose excretion in diabetes 60 13 Estimation of Blood Glucose ...

To Determine the Blood Glucose Levels by Folin and Wu Method^14 14.1 Principle........................................ The test ...

16.5 Procedure Take nine test tubes in duplicate, and label them as blank, S 1
S 7 (standard) and T (test). Add standard glucos ...

To Perform Glucose Tolerance Test 15 The ability of a person to metabolize a given glucose is called glucose tolerance. To asses ...

15.3 Principle........................................ The estimation of plasma glucose is based on the principle as explained f ...

Reagents Blank S 1 S^2 S^3 S^4 S^5 T^1 (fasting) T^2 (30 min) T^3 (60 min) T 4 (90 min) T^5 (120 min) T^2 (150 min) Phosphate bu ...

15.6 Calculations Plot a graph between amount of glucose at x-axis and absorbance at y-axis. Extrap- olate glucose amount of the ...

Estimation of Urea in Serum and Urine 16 16.1 Theory Urea is the major nitrogen-containing metabolite product of protein catabol ...

16.3 Principle........................................ Urea reacts directly with diacetyl monoxime under strong acidic condition ...

Mix 35 ml of solution A with 35 ml of solution B, and make up to 500 ml with distilled water. Store in a brown bottle at room te ...